Dahal Ram Hari, Kim Jaisoo
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Jan;67(1):127-132. doi: 10.1099/ijsem.0.001582. Epub 2017 Feb 20.
An aerobic, Gram-stain-negative, catalase-positive and oxidase-negative, non-motile, non-spore-forming, rod-shaped, pink-pigmented bacterium designated strain R491T was isolated from soil. Flexirubin-type pigments were absent. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain R491T formed a lineage within the family Methylobacteriaceae of the phylum Proteobacteria that was distinct from various species of the genus Microvirga, including Microvirgaaerilata 5420S-16T (97.83 % 16S rRNA gene sequence similarity), Microvirga zambiensis WSM3693T (97.76 %), Microvirga flocculans ATCC BAA-817T (97.41 %), and Microvirga lupini Lut6T, Microvirga subterranea DSM 14364T, Microvirga vignae BR3299T, and Microvirga guangxiensis 25BT (96.99 %). The major polar lipids of strain R491T were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 71.2 %), C16 : 0 (12.0 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 4.7 %), and C18 : 0 (4.2 %). The DNA G+C content of strain R491T was 61.8 mol%. DNA-DNA hybridization values between strain R491T and other members of the genus Microvirga ranged from 27 to 57 %. On the basis of phenotypic, genotypic and phylogenetic analyses, strain R491T represents a novel species of the genus Microvirga, for which the name Microvirga soli sp. nov. is proposed. The type strain is R491T (=KEMB 9005-408T=KACC 18969T=NBRC 112417T).
从土壤中分离出一株需氧、革兰氏染色阴性、过氧化氢酶阳性、氧化酶阴性、无运动性、不形成芽孢、杆状、产粉红色色素的细菌，命名为R491T菌株。该菌株不存在柔韧菌素型色素。基于16S rRNA基因序列的系统发育分析表明，R491T菌株在变形菌门甲基杆菌科内形成了一个分支，与微小病毒属的各种物种不同，包括空气微小病毒5420S-16T（16S rRNA基因序列相似性为97.83%）、赞比亚微小病毒WSM3693T（97.76%）、絮凝微小病毒ATCC BAA-817T（97.41%），以及羽扇豆微小病毒Lut6T、地下微小病毒DSM 14364T、豇豆微小病毒BR3299T和广西微小病毒25BT（96.99%）。R491T菌株的主要极性脂类为磷脂酰胆碱、磷脂酰甘油和磷脂酰乙醇胺。主要细胞脂肪酸为总和特征8（C18:1ω7c和/或C18:1ω6c；71.2%）、C16:0（12.0%）、总和特征3（C16:1ω7c和/或C16:1ω6c；4.7%）和C18:0（4.2%）。R491T菌株的DNA G+C含量为61.8 mol%。R491T菌株与微小病毒属其他成员之间的DNA-DNA杂交值在27%至57%之间。基于表型、基因型和系统发育分析，R491T菌株代表微小病毒属的一个新物种，为此提出新名称土壤微小病毒（Microvirga soli sp. nov.）。模式菌株为R491T（=KEMB 9005-408T=KACC 18969T=NBRC 112417T）。
