Meng Yu-Chen, Liu Hong-Can, Yang Lei-Lei, Kang Ying-Qian, Zhou Yu-Guang, Cai Man
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Department of Microbiology, Guizhou Medical University, Guiyang 550004, PR China.
Int J Syst Evol Microbiol. 2016 Dec;66(12):5556-5561. doi: 10.1099/ijsem.0.001556. Epub 2016 Oct 9.
Two bacterial strains SZDIS-1-1T and GZDIS-1-1 were isolated from fermentation bed of a pigpen in Fujian Province, China. Cells were Gram-stain-positive, facultatively anaerobic, short rods without flagellum. Their nearest phylogenetic neighbours were Microbacterium amylolyticum N5T (16S rRNA gene sequence similarity 98.2 %), Microbacterium indicum BBH6T (97.9 %) and Microbacterium gubbeenense DPC 5286T (97.8 %) with the DNA-DNA hybridization values to strain SZDIS-1-1T as 20.0±1.2, 14.3±5.8 and 19.1±1.6 %, respectively. The DNA G+C content of the new isolates was 67.6-71.1 mol% and anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 were their predominant cellular fatty acids. These results were consistent with classification into the genus Microbacterium. However, cell-wall sugars and characteristic amino acid were rhamnose, glucose, galactose and ornithine. Major menaquinones were MK-11 and MK-10. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, glycolipids, unknown phospholipid and unknown lipids. These characteristics constructed a distinct profile of the two isolates. Therefore, based on polyphasic taxonomic characteristics, strains SZDIS-1-1T and GZDIS-1-1 represented a novel species of genus Microbacterium, for which the name Microbacterium sorbitolivorans sp. nov. is proposed, with strain SZDIS-1-1T (=CGMCC 1.15228T=DSM 103422T) as the type strain.
从中国福建省一个猪圈的发酵床中分离出两株细菌菌株SZDIS-1-1T和GZDIS-1-1。细胞革兰氏染色呈阳性，兼性厌氧，为无鞭毛的短杆菌。它们最近的系统发育邻居是解淀粉微杆菌N5T（16S rRNA基因序列相似性为98.2%）、印度微杆菌BBH6T（97.9%）和古宾微杆菌DPC 5286T（97.8%），与菌株SZDIS-1-1T的DNA-DNA杂交值分别为20.0±1.2%、14.3±5.8%和19.1±1.6%。新分离株的DNA G+C含量为67.6 - 71.1 mol%，anteiso-C15 : 0、anteiso-C17 : 0和iso-C16 : 0是其主要的细胞脂肪酸。这些结果与归入微杆菌属一致。然而，细胞壁糖类和特征性氨基酸是鼠李糖、葡萄糖、半乳糖和鸟氨酸。主要的甲基萘醌是MK-11和MK-10。极性脂质由双磷脂酰甘油、磷脂酰甘油、糖脂、未知磷脂和未知脂质组成。这些特征构成了这两个分离株的独特特征。因此，基于多相分类学特征，菌株SZDIS-1-1T和GZDIS-1-1代表微杆菌属的一个新物种，为此提出名为嗜山梨醇微杆菌（Microbacterium sorbitolivorans）的新物种，菌株SZDIS-1-1T（=CGMCC 1.15228T=DSM 103422T）为模式菌株。
