Nguyen Tuan Manh, Kim Jaisoo
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, 16227, Republic of Korea.
Thai Nguyen University of Agriculture and Forestry, Quyet Thang Commune, Thai Nguyen City, Vietnam.
J Microbiol. 2017 Aug;55(8):635-639. doi: 10.1007/s12275-017-7092-1. Epub 2017 Jul 4.
There are marked differences between wet and freeze-dried cells with regard to the identification of polar lipid components. The determination of the polar lipid composition of freeze-dried cells is well established. However, several approaches to identifying polar lipid components in wet cells have met with limited success owing to the presence of non-polar compounds in the extracts, resulting in a lipid composition with a narrow scope. In this study, we surveyed the lipid profiles of the wet biomasses of three Gram-positive (Microbacterium lacticum, Rhodococcus koreensis, and Streptomyces longwoodensis) and two Gram-negative (Pseudomonas aeruginosa and Novosphingobium capsulatum) bacteria; the results were comparable in quality to those obtained using a standard freeze-dried approach. Moreover, our improved method ensures simple lipid extraction. Overall, the results of the analysis showed minor lipid profile differences between the two approaches with regard to quantity, and lipid identification was consistent in both methods for all species.
在极性脂质成分的鉴定方面，湿细胞和冻干细胞之间存在显著差异。冻干细胞极性脂质组成的测定方法已经很成熟。然而，由于提取物中存在非极性化合物，几种鉴定湿细胞中极性脂质成分的方法取得的成功有限，导致脂质组成范围较窄。在本研究中，我们调查了三种革兰氏阳性菌（乳酸微杆菌、韩国红球菌和长木链霉菌）和两种革兰氏阴性菌（铜绿假单胞菌和荚膜新鞘氨醇菌）湿生物质的脂质谱；结果在质量上与使用标准冻干方法获得的结果相当。此外，我们改进的方法确保了脂质提取的简便性。总体而言，分析结果表明，两种方法在脂质数量方面的差异较小，并且两种方法对所有物种的脂质鉴定都是一致的。
