Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
Institute of Agri-resources and Regional Planning, CAAS, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005660.
A Gram-stain positive, facultatively anaerobic, motile rod-shaped strain, BY-33, was isolated from a soil sample obtained from the Kubuqi Desert, PR China. Phylogenetic analysis based on 16S rRNA gene sequence similarity revealed that strain BY-33 was most closely related to the genus , including CF5-4 (98.2 % similarity), '' HO-Ch2 (98.0 %), THG-T121 (97.6 %), '' 69B4 (97.5 %), M (97.3 %) and '' T26 (97.0 %). The strain grew at 0‒37 °C (optimum, 28-30 °C) and pH 6.0-11.0 (optimum, pH 9.0-10.0) and with 0‒8.0 % (w/v) NaCl (optimum, 3.0%) on tryptic soy agar. It had catalase activity, but no oxidase activity. The polar lipids of strain BY-33 contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and phosphatidylinositol mannosides. The major respiratory quinone of strain BY-33 was MK-10 (H). Its major fatty acids were anteiso-C, anteiso-C A and C. The genomic DNA G+C content of strain BY-33 was 73.0 mol% based on total genome calculations. The average nucleotide identity scores between the genomic sequences of strain BY-33 and the other species of the genus were found to be low (ANIm <85.0 %, ANIb <77.0 % and OrthoANIu <78.0 %). Furthermore, the digital DNA-DNA hybridization and average amino acid identity values between strain BY-33 and the closely related species ranged from 20.5 to 21.0% and from 62.2 to 72.2 %, respectively. Based on the results of phylogenetic, phenotypic, genotypic and chemotaxonomic analyses, it is concluded that strain BY-33 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is BY-33 (=CGMCC 1.17460=KCTC 49362).
一株革兰氏阳性、兼性厌氧、能动的杆菌菌株 BY-33 是从中国库布其沙漠的土壤样本中分离得到的。基于 16S rRNA 基因序列相似性的系统发育分析表明，菌株 BY-33 与 属最为密切相关，包括 CF5-4（98.2%相似性）、'' HO-Ch2（98.0%）、THG-T121（97.6%）、'' 69B4（97.5%）、M（97.3%）和'' T26（97.0%）。该菌株在 0-37°C（最佳温度为 28-30°C）和 pH6.0-11.0（最佳 pH9.0-10.0）条件下生长，并在胰蛋白胨大豆琼脂上含有 0-8.0%（w/v）NaCl（最佳浓度为 3.0%）。它具有过氧化氢酶活性，但没有氧化酶活性。菌株 BY-33 的极性脂含有双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇和磷脂酰肌醇甘露糖苷。菌株 BY-33 的主要呼吸醌是 MK-10（H）。其主要脂肪酸为 anteiso-C、anteiso-C A 和 C。基于全基因组计算，菌株 BY-33 的基因组 DNA G+C 含量为 73.0mol%。菌株 BY-33 与属内其他种的基因组序列之间的平均核苷酸同一性评分发现较低（ANI<85.0%、ANIb<77.0%和 OrthoANIu<78.0%）。此外，菌株 BY-33 与亲缘关系密切的种之间的数字 DNA-DNA 杂交和平均氨基酸同一性值分别为 20.5-21.0%和 62.2-72.2%。基于系统发育、表型、基因型和化学分类分析的结果，得出结论，菌株 BY-33 代表 属中的一个新种，建议将其命名为 sp. nov.。模式菌株为 BY-33（=CGMCC 1.17460=KCTC 49362）。
