Hu Fengwang, Zhai Nana, Gao Wen, Wu Pei, Luo Yuanyuan, Pan Defeng, Liu Yang, Li Dongye
Institute of Cardiovascular Disease, Xuzhou Medical University Xuzhou, Jiangsu, China.
Department of Cardiology, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China.
PLoS One. 2016 Dec 1;11(12):e0167631. doi: 10.1371/journal.pone.0167631. eCollection 2016.
Coronary artery disease is a growing public health problem and a major cause of morbidity and mortality. Experimental animal models provide valuable tools for studying myocardial ischemia reperfusion (I/R) injury in vivo.
The purpose of this study was to describe a new method (outer balloon ligation) to induce myocardial I/R injury in mice.
Ninety-nine male C57BL/6J mice were randomly divided into three groups: sham group, classic method group (I/R-C) and the new method group (I/R-N). The surgical procedure and recovery time were recorded. The levels of TNF-α, IL-6, cTnT and LDH were detected by ELISA kits. Hematoxylin-eosin staining was applied to assess neutrophil infiltration. Moreover, surgical survival, myocardial infarction areas, and cardiac function measurements were also recorded.
The reperfusion operation time in the I/R-N group were markedly less than the I/R-C group (14.73±2.86 vs. 168.60±33.01 sec, p <0.0001). Similarly, the recovery time in I/R-N group was shorter than the I/R-C group (45.39±15.39 vs. 101.70±19.33 min, p <0.0001). The levels of TNF-α and IL-6 in I/R-N group were also markedly lower than in I/R-C group (136.5±22.21 vs. 170.5±24.79 pg/ml, p <0.05 and 100.3±23.74 vs. 144.40±22.24 pg/ml, p <0.001). Compared I/R-N group with I/R-C group, the levels of neutrophil infiltration, cTnT and LDH had no significant differences. Surgical survival rate was 96.7% in the I/R-N group, which was significantly improved compared to the rate of 80% in the I/R-C group. However, there were no significant differences in the areas of myocardial infarction and cardiac function between the two groups.
Compared with the classic method, our new method of inducing myocardial I/R injury has higher efficiency and less tissue damage in mice, but achieves the same modeling effects.
冠状动脉疾病是一个日益严重的公共卫生问题，也是发病和死亡的主要原因。实验动物模型为体内研究心肌缺血再灌注（I/R）损伤提供了有价值的工具。
本研究旨在描述一种诱导小鼠心肌I/R损伤的新方法（外球囊结扎法）。
将99只雄性C57BL/6J小鼠随机分为三组：假手术组、经典方法组（I/R-C）和新方法组（I/R-N）。记录手术过程和恢复时间。采用酶联免疫吸附测定试剂盒检测肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、心肌肌钙蛋白T（cTnT）和乳酸脱氢酶（LDH）水平。应用苏木精-伊红染色评估中性粒细胞浸润情况。此外，还记录手术存活率、心肌梗死面积和心功能测量结果。
I/R-N组的再灌注操作时间明显短于I/R-C组（14.73±2.86秒对168.60±33.01秒，p<0.0001）。同样，I/R-N组的恢复时间也短于I/R-C组（45.39±15.39分钟对101.70±19.33分钟，p<0.0001）。I/R-N组TNF-α和IL-6水平也明显低于I/R-C组（136.5±22.21皮克/毫升对170.5±24.79皮克/毫升，p<0.05；100.3±23.74皮克/毫升对144.40±22.24皮克/毫升，p<0.001）。I/R-N组与I/R-C组相比，中性粒细胞浸润、cTnT和LDH水平无显著差异。I/R-N组手术存活率为96.7%，与I/R-C组的80%相比有显著提高。然而，两组之间心肌梗死面积和心功能无显著差异。
与经典方法相比，我们诱导小鼠心肌I/R损伤的新方法效率更高，组织损伤更小，但建模效果相同。
