Ling Irving Tc, Rochard Lucie, Liao Eric C
Center for Regenerative Medic ine, Massachusetts General Hospital, Shriners Hospital for Children, Harvard Medical School, Boston, MA 02114, USA; School of Medicine, Veterinary and Life Sciences, Glasgow University, UK.
Center for Regenerative Medic ine, Massachusetts General Hospital, Shriners Hospital for Children, Harvard Medical School, Boston, MA 02114, USA.
Dev Biol. 2017 Jan 15;421(2):219-232. doi: 10.1016/j.ydbio.2016.11.016. Epub 2016 Nov 29.
Formation of the mandible requires progressive morphologic change, proliferation, differentiation and organization of chondrocytes preceding osteogenesis. The Wnt signaling pathway is involved in regulating bone development and maintenance. Chondrocytes that are fated to become bone require Wnt to polarize and orientate appropriately to initiate the endochondral ossification program. Although the canonical Wnt signaling has been well studied in the context of bone development, the effects of non-canonical Wnt signaling in regulating the timing of cartilage maturation and subsequent bone formation in shaping ventral craniofacial structure is not fully understood.. Here we examined the role of the non-canonical Wnt signaling pathway (wls, gpc4, wnt5b and wnt9a) in regulating zebrafish Meckel's cartilage maturation to the onset of osteogenic differentiation. We found that disruption of wls resulted in a significant loss of craniofacial bone, whereas lack of gpc4, wnt5b and wnt9a resulted in severely delayed endochondral ossification. This study demonstrates the importance of the non-canonical Wnt pathway in regulating coordinated ventral cartilage morphogenesis and ossification.
下颌骨的形成需要在成骨之前软骨细胞进行渐进性的形态变化、增殖、分化和组织化。Wnt信号通路参与调节骨骼的发育和维持。注定要形成骨骼的软骨细胞需要Wnt来进行适当的极化和定向，以启动软骨内成骨程序。尽管经典Wnt信号通路在骨骼发育方面已得到充分研究，但非经典Wnt信号在调节软骨成熟时间以及随后在塑造腹侧颅面结构中的骨形成方面的作用尚未完全明确。在此，我们研究了非经典Wnt信号通路（wls、gpc4、wnt5b和wnt9a）在调节斑马鱼梅克尔软骨成熟至成骨分化开始过程中的作用。我们发现，wls的破坏导致颅面骨显著缺失，而缺乏gpc4、wnt5b和wnt9a则导致软骨内成骨严重延迟。这项研究证明了非经典Wnt通路在调节腹侧软骨协调形态发生和骨化中的重要性。
