Laurie D, Manson A J, Rowell F J, Seviour J
School of Pharmaceutical and Chemical Sciences, Sunderland Polytechnic, Sunderland, UK.
Clin Chim Acta. 1989 Aug 15;183(2):183-95. doi: 10.1016/0009-8981(89)90334-3.
We describe a competitive inhibition ELISA technique, with a visual end-point, to detect free morphine in blood or urine. It has a sensitivity of 2 X 10(-7) mol/l using 5 microliter samples. No significant cross-reactivity was observed with other opiate derivatives. The assay has applications as a specific screen for morphine in drug abusers, or to study the metabolism of the drug in the body (as the metabolite, morphine-3-glucuronide, does not cross-react significantly with morphine in the assay).
我们描述了一种具有可视终点的竞争性抑制酶联免疫吸附测定（ELISA）技术，用于检测血液或尿液中的游离吗啡。使用5微升样本时，其灵敏度为2×10（-7）摩尔/升。未观察到与其他阿片类衍生物有明显交叉反应。该测定法可作为对药物滥用者体内吗啡的特异性筛查，或用于研究药物在体内的代谢（因为代谢物吗啡-3-葡糖苷酸在该测定中与吗啡无明显交叉反应）。
