Liang Shuang, Xu Xuan-Wei, Zhao Xiao-Feng, Hou Zhi-Guang, Wang Xin-Hong, Lu Zhong-Bin
College of Resources and Environment Science, Jilin Agricultural University, Changchun, Jilin, 130118, PR China.
Ginseng and Antler Products Testing Center of the Ministry of Agricultural PRC, Jilin Agricultural University, Changchun, Jilin 130118, PR China.
Pestic Biochem Physiol. 2016 Nov;134:63-72. doi: 10.1016/j.pestbp.2016.04.005. Epub 2016 Apr 20.
Panax ginseng C.A. Meyer is a valuable herb in China that has also gained popularity in the West because of its pharmacological properties. The constituents isolated and characterized in ginseng stems include ginsenosides, fatty acids, amino acids, volatile oils, and polysaccharides. In this study, the effects of fungicide azoxystrobin applied on antioxidant enzyme activity and ginsenosides content in ginseng stems was studied by using Panax ginseng C. A. Mey. cv. (the cultivar of Ermaya) under natural environmental conditions. The azoxystrobin formulation (25% SC) was sprayed three times on ginseng plants at different doses (150ga.i./ha and 225ga.i./ha), respectively. Two new fatty acids esters (ethyl linoleate and methyl linolenate) were firstly detected in ginseng stems by the application of azoxystrobin as foliar spray. The results indicated that activities of enzymatic antioxidants, the content of ginsenosides and two new fatty acids esters in ginseng stems in azoxystrobin-treated plants were increased. Azoxystrobin treatments to ginseng plants at all growth stages suggest that the azoxystrobin-induced delay of senescence is due to an enhanced antioxidant enzyme activity protecting the plants from harmful active oxygen species (AOS). The activity of superoxide dismutase (SOD) in azoxystrobin-treated plants was about 1-3 times higher than that in untreated plants. And the effects was more significant (P=0.05) when azoxystrobin was applied at dose of 225ga.i./ha. This work suggests that azoxystrobin plays an important role in delaying of senescence by changing physiological and biochemical indicators and increasing ginsenosides content in ginseng stems.
人参是中国一种珍贵的草药，因其药理特性在西方也颇受欢迎。人参茎中分离和鉴定出的成分包括人参皂苷、脂肪酸、氨基酸、挥发油和多糖。本研究在自然环境条件下，以人参品种二马牙为材料，研究了杀菌剂嘧菌酯对人参茎抗氧化酶活性和人参皂苷含量的影响。分别以不同剂量（150克有效成分/公顷和225克有效成分/公顷）对人参植株喷施3次嘧菌酯制剂（25%悬浮剂）。通过叶面喷施嘧菌酯，首次在人参茎中检测到两种新的脂肪酸酯（亚油酸乙酯和亚麻酸甲酯）。结果表明，嘧菌酯处理的人参茎中酶促抗氧化剂活性、人参皂苷含量及两种新脂肪酸酯含量均有所增加。在人参植株的所有生长阶段进行嘧菌酯处理表明，嘧菌酯诱导的衰老延迟是由于抗氧化酶活性增强，保护植株免受有害活性氧（AOS）的伤害。嘧菌酯处理植株中超氧化物歧化酶（SOD）活性比未处理植株高约1 - 3倍。当嘧菌酯以225克有效成分/公顷的剂量施用时，效果更显著（P = 0.05）。这项工作表明，嘧菌酯通过改变生理生化指标和增加人参茎中人参皂苷含量，在延缓衰老方面发挥重要作用。
