Tammina Sai Kumar, Mandal Badal Kumar, Ranjan Shivendu, Dasgupta Nandita
Trace Elements Speciation Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India.
Trace Elements Speciation Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India.
J Photochem Photobiol B. 2017 Jan;166:158-168. doi: 10.1016/j.jphotobiol.2016.11.017. Epub 2016 Nov 25.
Different sized tetragonal tin oxide nanoparticles (SnO NPs) were synthesized using Piper nigrum seed extract at three different calcination temperatures (300, 500, 900°C) and these nanoparticles (NPs) were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), dynamic light scattering (DLS) and Fourier transform infrared spectrophotometry (FT-IR). The optical properties were studied using UV-Vis and photoluminescence (PL) spectrophotometers. The generation of reactive oxygen species (ROS) was monitored by using a fluorescence spectrophotometer and fluorescence microscope. The cytotoxicity of the synthesized SnO NPs was checked against the colorectal (HCT116) and lung (A549) cancer cell lines and the study results show that SnO NPs were toxic against cancer cell lines depending on their size and dose. IC values of SnO NPs having average particle sizes of 8.85±3.5, 12.76±3.9 and 29.29±10.9nm are 165, 174 and 208μgL against HCT116, while these values are 135, 157 and 187μgL against A549 carcinoma cell lines, respectively. The generated ROS were responsible for the cytotoxicity of SnO NPs to the studied cancer cells and smaller size NPs generated more ROS and hence showed higher cytotoxicity over larger size NPs. The results of this study suggest that the synthesized stable nanoparticles could be a potent therapeutic agent towards cancerous cell lines.
使用胡椒籽提取物在三种不同的煅烧温度（300、500、900°C）下合成了不同尺寸的四方氧化锡纳米颗粒（SnO NPs），并通过透射电子显微镜（TEM）、X射线衍射（XRD）、动态光散射（DLS）和傅里叶变换红外光谱法（FT-IR）对这些纳米颗粒进行了表征。使用紫外可见分光光度计和光致发光（PL）分光光度计研究了光学性质。通过荧光分光光度计和荧光显微镜监测活性氧（ROS）的产生。检测了合成的SnO NPs对结肠癌细胞系（HCT116）和肺癌细胞系（A549）的细胞毒性，研究结果表明，SnO NPs对癌细胞系具有毒性，这取决于它们的尺寸和剂量。平均粒径为8.85±3.5、12.76±3.9和29.29±10.9nm的SnO NPs对HCT116的IC值分别为165、174和208μg/L，而对A549癌细胞系的这些值分别为135、157和187μg/L。产生的ROS是SnO NPs对所研究癌细胞具有细胞毒性的原因，较小尺寸的纳米颗粒产生更多的ROS，因此比较大尺寸的纳米颗粒表现出更高的细胞毒性。本研究结果表明，合成的稳定纳米颗粒可能是一种有效的癌细胞系治疗剂。
