Choi Geun Joo, Kang Hyun, Kim Won Joong, Kwon Ji Wung, Kim Beom Gyu, Choi Yoo Shin, Cha Young Joo, Ko Jin Soo
Department of Anesthesiology and Pain Medicine, College of Medicine, Chung-Ang University, Seoul, Korea.
Department of Anesthesiology and Pain Medicine, College of Medicine, Chung-Ang University, Seoul, Korea.
J Surg Res. 2016 Nov;206(1):223-230. doi: 10.1016/j.jss.2016.08.005. Epub 2016 Aug 9.
The purpose of this study was to evaluate the analgesic effect of Rubus occidentalis extract (ROE) in a rat model of incisional pain. The involved mechanisms and proinflammatory cytokine response were also examined.
To investigate the analgesic effect, rats were intraperitoneally administered with normal saline or various doses of ROE before or after a plantar incision. To evaluate the involved mechanism, rats were intraperitoneally administered yohimbine, dexmedetomidine, prazosin, naloxone, atropine, or mecamylamine after a plantar incision; ROE was then administered intraperitoneally. The mechanical withdrawal threshold (MWT) was tested with von Frey filaments at various time points. To determine the inflammatory response, serum levels of interleukin (IL)-1β or IL-6 were measured.
The MWTs significantly increased at 15 min after postincisional administration of 300 mg/kg ROE when compared with those in the control group. This elevation was observed for up to 45 min. Overall, MWTs increased in proportion to ROE dosage; however, ROEs administered before the incision produced no significant change in the MWT. The analgesic effect of ROE was significantly antagonized by mecamylamine, naloxone, and yohimbine, and agonized by dexmedetomidine. Administration of ROE inhibited the postincisional increase in serum IL-1β and IL-6.
Intraperitoneal administration of ROE after surgery induces antinociceptive effects in a rat model of postoperative pain, and its effects on mechanical hyperalgesia may be associated with α-adrenergic, nicotinic cholinergic, and opioid receptors.
本研究旨在评估西方悬钩子提取物（ROE）在大鼠切口疼痛模型中的镇痛效果。同时还研究了其相关机制及促炎细胞因子反应。
为研究镇痛效果，在大鼠足底切口前或后腹腔注射生理盐水或不同剂量的ROE。为评估相关机制，在大鼠足底切口后腹腔注射育亨宾、右美托咪定、哌唑嗪、纳洛酮、阿托品或美加明；然后腹腔注射ROE。在不同时间点用von Frey细丝测试机械性撤针阈值（MWT）。为确定炎症反应，检测血清白细胞介素（IL）-1β或IL-6水平。
与对照组相比，术后给予300mg/kg ROE后15分钟时MWT显著升高。这种升高持续长达45分钟。总体而言，MWT随ROE剂量增加而升高；然而，切口前给予ROE对MWT无显著影响。ROE的镇痛作用被美加明、纳洛酮和育亨宾显著拮抗，被右美托咪定增强。给予ROE可抑制术后血清IL-1β和IL-6的升高。
术后腹腔注射ROE可在大鼠术后疼痛模型中诱导抗伤害感受作用，其对机械性痛觉过敏的作用可能与α-肾上腺素能、烟碱型胆碱能和阿片受体有关。
