Choi Geun Joo, Kang Hyun, Lee Jun Mo, Baek Chong Wha, Jung Yong Hun, Woo Young Cheol, Do Jae Hyuk, Ko Jin Soo
Department of Anesthesiology and Pain Medicine, Chung-Ang University College of Medicine, Seoul, Korea.
Department of Internal Medicine, Chung-Ang University College of Medicine, Seoul, Korea.
Korean J Pain. 2020 Oct 1;33(4):326-334. doi: 10.3344/kjp.2020.33.4.326.
In this study, we sought to evaluate whether systemic propentofylline (PPF) has antiallodynic effects in a rat model of postoperative pain, and to assess the mechanism involved.
After plantar incision, rats were intraperitoneally injected with various doses of PPF to evaluate its antiallodynic effect. To investigate the involved mechanism, rats were intraperitoneally injected with yohimbine, dexmedetomidine, prazosin, naloxone, atropine or mecamylamine, following the incision of the rat hind paws, and then PPF was administered intraperitoneally. The mechanical withdrawal threshold (MWT) was evaluated using von Frey filaments at various time points and serum levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 were measured to determine the inflammatory response level.
MWT was significantly increased after intraperitoneal injection of 30 mg/kg of PPF when compared with the control group. Injection of PPF and yohimbine, atropine or mecamylamine showed significant decreases in the MWT, while injection of PPF and dexmedetomidine showed a significant increase. Systemic administration of PPF inhibited the post-incisional increase in serum level of TNF-α and IL-1β.
Systemic administration of PPF following surgery presented antiallodynic effects in a rat model of postoperative pain. The antiallodynic effects against mechanical allodynia could be mediated by α-adrenergic and cholinergic receptors.
在本研究中，我们试图评估全身性丙戊茶碱（PPF）在大鼠术后疼痛模型中是否具有抗痛觉过敏作用，并评估其涉及的机制。
在足底切开后，给大鼠腹腔注射不同剂量的PPF以评估其抗痛觉过敏作用。为了研究其涉及的机制，在大鼠后爪切开后，给大鼠腹腔注射育亨宾、右美托咪定、哌唑嗪、纳洛酮、阿托品或美加明，然后腹腔注射PPF。在不同时间点使用von Frey细丝评估机械性撤针阈值（MWT），并测量血清肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β和IL-6水平以确定炎症反应水平。
与对照组相比，腹腔注射30mg/kg的PPF后MWT显著增加。注射PPF与育亨宾、阿托品或美加明后MWT显著降低，而注射PPF与右美托咪定后MWT显著增加。全身性给予PPF可抑制切开后血清TNF-α和IL-1β水平的升高。
术后全身性给予PPF在大鼠术后疼痛模型中呈现抗痛觉过敏作用。对机械性痛觉过敏的抗痛觉过敏作用可能由α-肾上腺素能和胆碱能受体介导。
