基于DNA纳米结构修饰磁珠的溶藻弧菌电位适体传感
Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads.
作者信息
Zhao Guangtao, Ding Jiawang, Yu Han, Yin Tanji, Qin Wei
机构信息
Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai 264003, Shandong, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
出版信息
Sensors (Basel). 2016 Dec 2;16(12):2052. doi: 10.3390/s16122052.
A potentiometric aptasensing assay that couples the DNA nanostructure-modified magnetic beads with a solid-contact polycation-sensitive membrane electrode for the detection of is herein described. The DNA nanostructure-modified magnetic beads are used for amplification of the potential response and elimination of the interfering effect from a complex sample matrix. The solid-contact polycation-sensitive membrane electrode using protamine as an indicator is employed to chronopotentiometrically detect the change in the charge or DNA concentration on the magnetic beads, which is induced by the interaction between and the aptamer on the DNA nanostructures. The present potentiometric aptasensing method shows a linear range of 10-100 CFU mL with a detection limit of 10 CFU mL, and a good specificity for the detection of . This proposed strategy can be used for the detection of other microorganisms by changing the aptamers in the DNA nanostructures.
本文描述了一种电位适配体传感分析方法，该方法将DNA纳米结构修饰的磁珠与固体接触聚阳离子敏感膜电极相结合用于检测。DNA纳米结构修饰的磁珠用于放大电位响应并消除复杂样品基质的干扰效应。使用鱼精蛋白作为指示剂的固体接触聚阳离子敏感膜电极用于计时电位法检测磁珠上电荷或DNA浓度的变化，这种变化是由与DNA纳米结构上的适配体之间的相互作用引起的。当前的电位适配体传感方法显示出10 - 100 CFU/mL的线性范围，检测限为10 CFU/mL，并且对检测具有良好的特异性。通过改变DNA纳米结构中的适配体，该提出的策略可用于检测其他微生物。
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