Ding Jiawang, Lei Jiahong, Ma Xia, Gong Jun, Qin Wei
Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS , Yantai, Shandong 264003, P. R. China.
Anal Chem. 2014 Oct 7;86(19):9412-6. doi: 10.1021/ac502335g. Epub 2014 Sep 24.
Exposure to pathogens in recreational or drinking water is a serious public health concern. It is important to rapidly determine and identify trace levels of pathogens in real environmental samples. We report here on a label-free potentiometric aptasensor for rapid, sensitive, and selective detection of Listeria monocytogenes (LM), a pathogen widely distributed in the environment. An aptamer binds specifically to internalin A, a surface protein present in LM cells. The target-binding event prevents the aptamer from electrostatically interacting with protamine, which can be sensitively detected using a polycation-sensitive membrane electrode. Using this method, LM can be detected down to 10 CFU mL(-1). Coupled to an online filtration system, the bioassay has been evaluated with spiked coastal seawater samples and shows good recovery and high accuracy. This work demonstrates the possibility of developing potentiometric aptasensors for determination and identification of various bacteria in environmental samples.
在娱乐用水或饮用水中接触病原体是一个严重的公共卫生问题。快速确定和识别实际环境样本中的痕量病原体非常重要。我们在此报告一种无标记电位适体传感器，用于快速、灵敏和选择性地检测单核细胞增生李斯特菌（LM），这是一种广泛分布于环境中的病原体。一种适体特异性结合内化素A，内化素A是LM细胞中存在的一种表面蛋白。靶标结合事件可防止适体与鱼精蛋白发生静电相互作用，而鱼精蛋白可使用聚阳离子敏感膜电极进行灵敏检测。使用这种方法，可检测到低至10 CFU mL(-1)的LM。与在线过滤系统相结合，该生物测定法已用于加标沿海海水样本的评估，并显示出良好的回收率和高精度。这项工作证明了开发电位适体传感器用于测定和识别环境样本中各种细菌的可能性。
