Maharaj Niren Ray, Ramkaran Prithiksha, Pillay Siddharthiya, Chuturgoon Anil Amichund
Department of Obstetrics and Gynaecology, Prince Mshiyeni Memorial Hospital, Durban, South Africa.
Discipline of Medical Biochemistry and Chemical Pathology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Howard College Campus, George Campbell Building - South Entrance, 3rd Floor, Kind George V Avenue, Durban, South Africa.
BMC Med Genet. 2016 Dec 5;17(1):92. doi: 10.1186/s12881-016-0353-8.
Preeclampsia (PE) and HIV/AIDS present a major health challenge globally. South Africa has the highest disease burden of both HIV/AIDS and PE in the world. Despite extensive research, the pathophysiology of these conditions is not completely understood, however a genetic predisposition in women may affect susceptibility. MiRNA-27a regulates adipogenesis and glucose metabolism. A single nucleotide polymorphism (SNP) in miRNA-27a (rs895819T > C) has shown to have disparate effects in various populations. This study investigated the frequency of rs895819 in pregnant normotensive and preeclamptic Black South African (SA) women.
Enrollment into the study included: normotensive (n = 95; 45 HIV+; 80 analysed for rs895819T > C, age range: 16-46 years) and PE patients (n = 98; 45 HIV+; 56 analysed for rs895819T > C), age range: 16-42 years). DNA was isolated from peripheral blood mononuclear cells (PBMC). Genotyping of miRNA-27a rs895819 was detected using a TaqMan® SNP Genotyping assay.
We did not find a significant association of miR-27a polymorphism with PE susceptibility in our data. However, in the subgroup analysis (based in HIV status), the variant genotypes (TC/CC) were associated with higher body mass index (BMI) among PE women (32.57 vs. 29.25, p = 0.064), significantly in the presence of HIV infection (33.47 vs. 27.8, p = 0.005).
The results of this study suggests that miR-27a rs895819 may not be associated with PE susceptibility; however, the miR-27a TC/CC genotype increases susceptibility to elevated BMI in PE, which may be significantly influenced by co-morbid HIV infection among pregnant women on HAART.
子痫前期（PE）和艾滋病毒/艾滋病是全球主要的健康挑战。南非是世界上艾滋病毒/艾滋病和子痫前期疾病负担最高的国家。尽管进行了广泛研究，但这些病症的病理生理学尚未完全了解，不过女性的遗传易感性可能会影响易感性。微小RNA-27a调节脂肪生成和葡萄糖代谢。微小RNA-27a中的单核苷酸多态性（SNP）（rs895819T>C）在不同人群中显示出不同的影响。本研究调查了南非黑人正常血压孕妇和子痫前期孕妇中rs895819的频率。
纳入研究的对象包括：正常血压者（n = 95；45名艾滋病毒阳性；80名分析了rs895819T>C，年龄范围：16 - 46岁）和子痫前期患者（n = 98；45名艾滋病毒阳性；56名分析了rs895819T>C），年龄范围：16 - 42岁。从外周血单核细胞（PBMC）中分离DNA。使用TaqMan®SNP基因分型检测法检测微小RNA-27a rs895819的基因分型。
在我们的数据中，未发现miR-27a多态性与子痫前期易感性之间存在显著关联。然而，在亚组分析（基于艾滋病毒感染状况）中，变异基因型（TC/CC）与子痫前期女性中较高的体重指数（BMI）相关（32.57对29.25，p = 0.064），在存在艾滋病毒感染时显著相关（33.47对27.8，p = 0.005）。
本研究结果表明，miR-27a rs895819可能与子痫前期易感性无关；然而，miR-27a的TC/CC基因型增加了子痫前期患者BMI升高的易感性，这可能受到接受高效抗逆转录病毒治疗的孕妇中合并艾滋病毒感染的显著影响。
