Ghaedi H, Tabasinezhad M, Alipoor B, Shokri F, Movafagh A, Mirfakhraie R, Omrani M D, Masotti A
Medical Genetics Department, School of Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Medical Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran.
J Endocrinol Invest. 2016 Oct;39(10):1187-93. doi: 10.1007/s40618-016-0499-4. Epub 2016 Jun 14.
The study was aimed at investigating the association between hsa-mir-27a polymorphism rs895819 (T/C) and type 2 diabetes mellitus (T2DM) susceptibility in a large Iranian cohort.
In this case-control study, the investigated population consisted of T2DM patients (n = 204) and sex- and age-matched controls (n = 209). We used the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) for genotyping.
We observed significant differences between T2DM patients and controls for weight (p = 0.002), BMI (p < 0.001), systolic blood pressure (p < 0.001), diastolic blood pressure (p < 0.001), fasting plasma glucose (p < 0.001), triglyceride (p = 0.004) and LDL cholesterol (p = 0.051). Moreover, we found that genotype distributions were significantly different between groups (p < 0.05) and that the rs895819-C allele is more frequent in controls (p = 0.030, OR = 0.72, 95 % CI 0.53-0.97).
Our study shows that rs895819 in hsa-mir-27a is associated with T2DM susceptibility and that the C allele conveyed a protective role against T2DM. Larger multicentric and specific functional studies will be necessary to obtain a deeper comprehension of the role of rs895819 and hsa-mir-27a and how they are involved in the development of diabetes.
本研究旨在调查伊朗一个大型队列中hsa - mir - 27a基因多态性rs895819（T/C）与2型糖尿病（T2DM）易感性之间的关联。
在这项病例对照研究中，被调查人群包括T2DM患者（n = 204）以及性别和年龄匹配的对照组（n = 209）。我们使用聚合酶链反应和限制性片段长度多态性（PCR - RFLP）进行基因分型。
我们观察到T2DM患者与对照组在体重（p = 0.002）、体重指数（BMI）（p < 0.001）、收缩压（p < 0.001）、舒张压（p < 0.001）、空腹血糖（p < 0.001）、甘油三酯（p = 0.004）和低密度脂蛋白胆固醇（p = 0.051）方面存在显著差异。此外，我们发现两组之间的基因型分布存在显著差异（p < 0.05），并且rs895819 - C等位基因在对照组中更为常见（p = 0.030，OR = 0.72，95% CI 0.53 - 0.97）。
我们的研究表明，hsa - mir - 27a中的rs895819与T2DM易感性相关，并且C等位基因对T2DM具有保护作用。需要进行更大规模的多中心和特定功能研究，以更深入地理解rs895819和hsa - mir - 27a的作用以及它们如何参与糖尿病的发生发展。
