黑曲霉NRRL-3葡萄糖氧化酶基因的克隆与DNA序列分析
Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3.
作者信息
Kriechbaum M, Heilmann H J, Wientjes F J, Hahn M, Jany K D, Gassen H G, Sharif F, Alaeddinoglu G
机构信息
Institut für Biochemie, Technische Hochschule Darmstadt, FRG.
出版信息
FEBS Lett. 1989 Sep 11;255(1):63-6. doi: 10.1016/0014-5793(89)81061-0.
A cDNA library from Aspergillus niger strain NRRL-3 enriched in sequences glucose oxidase was constructed. An 800 bp cDNA clone isolated from this library was used to screen 12,000 recombinant phages from an EMBL3 genomic library. A 15 kbp DNA segment isolated from this library contained the 1815 bp structural gene for glucose oxidase as well as a short 5'- and a longer 3'-noncoding region. The deduced protein sequence was verified by partial peptide sequencing.
构建了一个来自黑曲霉NRRL-3菌株且富含葡萄糖氧化酶序列的cDNA文库。从该文库中分离出一个800 bp的cDNA克隆，用于筛选来自EMBL3基因组文库的12,000个重组噬菌体。从该文库中分离出的一个15 kbp的DNA片段包含葡萄糖氧化酶的1815 bp结构基因以及一个短的5'非编码区和一个较长的3'非编码区。推导的蛋白质序列通过部分肽测序得到验证。
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