大麻哈鱼促黑素细胞激素编码的两个基因的结构

Structures of two genes coding for melanin-concentrating hormone of chum salmon.

作者信息

Takayama Y, Wada C, Kawauchi H, Ono M

机构信息

Department of Molecular Biology, School of Medicine, Kitasato University, Kanagawa, Japan.

出版信息

Gene. 1989 Aug 1;80(1):65-73. doi: 10.1016/0378-1119(89)90251-5.

DOI:10.1016/0378-1119(89)90251-5

PMID:2792771

Abstract

Two MCH genes coding for melanin-concentrating hormone (MCH) were isolated from a chum salmon liver DNA library and characterized. They were shown to be intronless genes with 0.63-kb exons, each of which commonly consisted of an about 80-bp 5'-untranslated region, a region coding for 132 amino acids (aa) MCH precursor protein and an approx. 160-bp 3'-untranslated region. About 20 bp upstream from the putative cap site, sequences were found corresponding to the TATA box. The two genes were 86% identical at the nucleotide sequence level. Sequences homologous to the chum salmon MCH genes were present in the genomes of other fish such as catfish, carp and Chinese grass carp, whereas no highly homologous sequence could be detected in other vertebrate genomes.

摘要

从银大麻哈鱼肝脏DNA文库中分离并鉴定了两个编码黑色素浓缩激素（MCH）的MCH基因。它们被证明是无内含子基因，外显子长度为0.63 kb，每个外显子通常由一个约80 bp的5'非翻译区、一个编码132个氨基酸（aa）的MCH前体蛋白的区域和一个约160 bp的3'非翻译区组成。在假定的帽位点上游约20 bp处，发现了与TATA盒对应的序列。这两个基因在核苷酸序列水平上的同源性为86%。鲶鱼、鲤鱼和草鱼等其他鱼类的基因组中存在与银大麻哈鱼MCH基因同源的序列，而在其他脊椎动物基因组中未检测到高度同源的序列。