Hoseinzadeh Taifeh, Ghanbarpour Reza, Rokhbakhsh-Zamin Farokh
Department of Biology, Kerman Branch, Islamic Azad University, Kerman, Iran.
Molecular Microbiology Department, Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran.
Iran J Microbiol. 2016 Jun;8(3):187-192.
Diarrheagenic (DEC) strains are a major cause of intestinal syndromes in the developing countries. The aim of this study was to determine the prevalence of enterotoxigenic (ETEC) and enteroinvasive (EIEC) in relation to phylogenetic background from patients with diarrhea.
A total of 110 isolates were obtained from diarrhea patients in Sirjan, southeast of Iran. The isolates were confirmed using biochemical and bacteriological tests. DNA of isolates was extracted by boiling method and checked for existence of ETEC ( and genes) and EIEC ( gene) pathotypes and also characterize the phylogenetic groups on the basis of presence or absence of the genes and an anonymous DNA fragment, TspE4. C2 by multiplex PCR.
Out of 110 isolates, 32 (29.09%) were positive for ETEC ( and genes) and 6 (5.45%) possessed EIEC ( gene) pathotypes. Isolates fall into four phylogenetic groups: A (39.09%), B1 (20%), B2 (15.45%) and D (25.45%). Phylotyping of isolates of DEC indicated they were distributed in four phylogenetic groups including A (12 isolates), B1 (7), B2 (9) and D (10).
In this study, the DEC isolates were segregated into different phylogenetic groups. The majority of isolates belonged to phylo-groups A and D.
致泻性大肠埃希菌（DEC）菌株是发展中国家肠道综合征的主要病因。本研究旨在确定腹泻患者中肠产毒性大肠埃希菌（ETEC）和肠侵袭性大肠埃希菌（EIEC）的流行情况及其系统发育背景。
从伊朗东南部锡尔詹的腹泻患者中总共分离出110株菌株。通过生化和细菌学检测对分离株进行鉴定。采用煮沸法提取分离株的DNA，检测ETEC（和基因）及EIEC（基因）致病型的存在情况，并根据基因和一个匿名DNA片段TspE4.C2的有无通过多重PCR对系统发育群进行鉴定。
在110株分离株中，32株（29.09%）ETEC（和基因）检测呈阳性，6株（5.45%）具有EIEC（基因）致病型。分离株分为四个系统发育群：A群（39.09%）、B1群（20%）、B2群（15.45%）和D群（25.45%）。DEC分离株的系统发育分型表明它们分布在四个系统发育群中，包括A群（12株）、B1群（7株）、B2群（9株）和D群（10株）。
在本研究中，DEC分离株被分为不同的系统发育群。大多数分离株属于A群和D群。
