Phylogenetic background of enterotoxigenic and enteroinvasive from patients with diarrhea in Sirjan, Iran.

BACKGROUND AND OBJECTIVES

Diarrheagenic (DEC) strains are a major cause of intestinal syndromes in the developing countries. The aim of this study was to determine the prevalence of enterotoxigenic (ETEC) and enteroinvasive (EIEC) in relation to phylogenetic background from patients with diarrhea.

MATERIALS AND METHODS

A total of 110 isolates were obtained from diarrhea patients in Sirjan, southeast of Iran. The isolates were confirmed using biochemical and bacteriological tests. DNA of isolates was extracted by boiling method and checked for existence of ETEC ( and genes) and EIEC ( gene) pathotypes and also characterize the phylogenetic groups on the basis of presence or absence of the genes and an anonymous DNA fragment, TspE4. C2 by multiplex PCR.

RESULTS

Out of 110 isolates, 32 (29.09%) were positive for ETEC ( and genes) and 6 (5.45%) possessed EIEC ( gene) pathotypes. Isolates fall into four phylogenetic groups: A (39.09%), B1 (20%), B2 (15.45%) and D (25.45%). Phylotyping of isolates of DEC indicated they were distributed in four phylogenetic groups including A (12 isolates), B1 (7), B2 (9) and D (10).

CONCLUSION

In this study, the DEC isolates were segregated into different phylogenetic groups. The majority of isolates belonged to phylo-groups A and D.