East Mediterr Health J. 2013 Jul;19(7):617-21.
Diarrhoeagenic Escherichia coli can be considered as the most important etiologic agents of diarrhoea in the Islamic Republic of Iran, particularly in children. This study determined the frequency of diarrhoeagenic E. coli isolates collected from children with acute diarrhoea (n = 50) and a control group (n = 50) at an Iranian referral paediatric centre during a 1-year period. Using multiplex PCR, diarrhoeagenic E. coli was identified in 90% ofthe case group and 20% of controls. Enterotoxigenic E coli was the most frequently identified pathotype in both groups (26% in cases; 10% in controls). Shiga toxin-producing E. coli was the second most isolated pathotype (17%), followed by enteroaggregative E. coli (12%). No enteroinvasive E. coli and enteropathogenic E. coli strains were recovered. More than 80% of isolates harboured the fimH gene. This high proportion of diarrhoeagenic E. coli and diversity of E. coli types highlights the need for enhanced surveillance of gastroenteritis agents in children in this country.
产肠毒性大肠杆菌可被视为伊朗伊斯兰共和国（尤其是儿童）腹泻的最重要病因。本研究在为期 1 年的时间内，对伊朗转诊儿科中心的急性腹泻患儿（n=50）和对照组（n=50）中采集的产肠毒性大肠杆菌分离株的频率进行了测定。采用多重 PCR 方法，在病例组的 90%和对照组的 20%中鉴定出产肠毒性大肠杆菌。肠毒素性大肠杆菌是两组中最常鉴定出的病原型（病例组 26%；对照组 10%）。产志贺毒素大肠杆菌是第二常见的分离株（17%），其次是聚集性大肠杆菌（12%）。未分离到侵袭性大肠杆菌和肠致病性大肠杆菌菌株。超过 80%的分离株携带 fimH 基因。如此高比例的产肠毒性大肠杆菌和大肠杆菌类型的多样性，突显了加强对该国儿童胃肠炎病原体监测的必要性。
