Immunoreactivity evaluation of a new recombinant chimeric protein against in the murine model.

BACKGROUND AND OBJECTIVES

Brucellosis is an important health problem in developing countries and no vaccine is available for the prevention of infection in humans. Because of clinically infectious diseases and their economic consequences in human and animals, designing a proper vaccine against is desirable. In this study, we evaluated the immune responses induced by a designed recombinant chimera protein in murine model.

MATERIALS AND METHODS

Three immunodominant antigens of have been characterized as potential immunogenic and protective antigens including: trigger factor (TF), Omp31 and Bp26 were fused together by EAAAK linkers to produce a chimera (structure were designed ), which was synthesized, cloned, and expressed in BL21 (DE3). The purification of recombinant protein was performed using Ni-NTA agarose. SDS-PAGE and anti-His antibody was used for confirmation purified protein (Western blot). BALB/c immunization was performed by purified protein and adjuvant, and sera antibody levels were measured by ELISA. otted.

RESULTS

SDS-PAGE and Western blotting results indicated the similarity of designing and experiments. ELISA result proved that the immunized sera of mice contain high levels of antibodies (IgG) against recombinant chimeric protein.

CONCLUSION

The recombinant chimeric protein could be a potential antigen candidate for the development of a subunit vaccine against