Liu Jia-Li, Zhao Min, Zhuo Ying, Chai Ya-Qin, Yuan Ruo
Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.
Chemistry. 2017 Feb 3;23(8):1853-1859. doi: 10.1002/chem.201604411. Epub 2017 Jan 16.
The intermolecular electrochemiluminescence resonance energy transfer (ECL-RET) between luminol and Ru(bpy) was studied extensively to achieve the sensitive bioanalysis owing to the perfect spectral overlap of the donor and acceptor, but it still suffers from the challenging issue of low energy-transfer efficiency. The intramolecular ECL-RET towards the novel ECL compound containing the donor of luminol and the acceptor of Ru(bpy) (mcpbpy) (Lum-Ru) was designed and investigated. With the high-efficient ECL-RET in one molecule, the highly intense ECL signal of Lum-Ru was obtained owing to the short path of energy transmission and less energy loss between luminol and Ru(bpy) (mcpbpy) . Lum-Ru was further applied to construct a signal-off electrochemiluminescence (ECL) aptasensor for ultrasensitive detection of a harsh carcinogen of Aflatoxin M1 (AFM1). This sensing platform also provides a significant boost for the trace detection of other biomolecules in clinical analysis.
由于供体和受体具有完美的光谱重叠，鲁米诺与钌（联吡啶）之间的分子间电化学发光共振能量转移（ECL-RET）被广泛研究以实现灵敏的生物分析，但它仍然面临能量转移效率低这一具有挑战性的问题。设计并研究了朝向含有鲁米诺供体和钌（联吡啶）（mcpbpy）受体的新型ECL化合物（Lum-Ru）的分子内ECL-RET。由于在一个分子中具有高效的ECL-RET，由于能量传输路径短且鲁米诺与钌（联吡啶）（mcpbpy）之间的能量损失较少，因此获得了Lum-Ru的高强度ECL信号。Lum-Ru进一步应用于构建用于超灵敏检测黄曲霉毒素M1（AFM1）这种剧毒致癌物的信号关闭型电化学发光（ECL）适配体传感器。该传感平台也为临床分析中其他生物分子的痕量检测提供了显著助力。
