Angrup A, Chaudhry R, Sharma S, Valavane A, Passi K, Padmaja K, Javed S, Dey A B, Dhawan B, Kabra S K
Department of Microbiology, All Institute of Medical Sciences, New Delhi, India.
Department of Medicine, All Institute of Medical Sciences, New Delhi, India.
Indian J Med Microbiol. 2016 Oct-Dec;34(4):539-543. doi: 10.4103/0255-0857.195353.
Legionella pneumophila is one of the important pathogen responsible for community -acquired pneumonia attributing for 1-5% of cases. Since early and accurate therapy reduces mortality, rapid and reliable diagnostic methods are needed. A total of 134 samples of blood, urine and respiratory tract fluids were collected. Blood was tested for IgG, IgM and IgA antibodies using commercially available kits. A total of 8 (6%) samples were found to be positive for L. pneumophila by quantitative reverse transcription polymerase chain reaction (qRT-PCR), compared to conventional PCR where 6 (4.4%) samples were positive. Serology was positive in a total of 32 (23%) cases though only 3 (2.2%) of the PCR-positive cases were positive by serology as well. These results suggest that real-time PCR can detect Legionella infection early in the course of the disease before serological response develops.
嗜肺军团菌是引起社区获得性肺炎的重要病原体之一，占病例的1%-5%。由于早期准确的治疗可降低死亡率，因此需要快速可靠的诊断方法。共采集了134份血液、尿液和呼吸道液体样本。使用市售试剂盒检测血液中的IgG、IgM和IgA抗体。通过定量逆转录聚合酶链反应（qRT-PCR），共发现8份（6%）样本的嗜肺军团菌呈阳性，而传统PCR检测中6份（4.4%）样本呈阳性。血清学检测共有32例（23%）呈阳性，但PCR阳性病例中只有3例（2.2%）血清学检测也呈阳性。这些结果表明，实时PCR可在疾病过程中血清学反应出现之前早期检测到军团菌感染。
