Valat J, Devau G, Dulon D, Sans A
INSERM U-254, Laboratoire de Neurophysiologie Sensorielle, U.S.T.L., Montpellier, France.
Hear Res. 1989 Jul;40(3):255-60. doi: 10.1016/0378-5955(89)90166-4.
Living sensory cells were isolated from the cristae ampullaris and macula utriculi of the guinea pig. Enzymatic and mechanical dissociation were used to obtain different populations of hair cells, the most predominant being type I cells. Their form varied: cell body of variable roundness, and neck and cilia of different lengths. The observation of many tilted cuticular plates supports the hypothesis of active mechanisms regulating mechanotransduction at the apex of these cells. Cell viability was verified by double fluorescent labeling (FDA-PI), which indicated that under correct conditions about 90% of the sensory cells could be maintained in vitro for several hours after dissociation. The detection of actin in the cuticular plate and cilia shows that the technique has various potential applications in morphological studies, and can contribute to investigations on the physiology of mammalian vestibular cells.
从豚鼠的壶腹嵴和椭圆囊斑中分离出活的感觉细胞。采用酶解和机械解离的方法获得不同群体的毛细胞，其中最主要的是I型细胞。它们的形态各异：细胞体的圆度不一，颈部和纤毛的长度也各不相同。对许多倾斜角质板的观察支持了调节这些细胞顶端机械转导的主动机制假说。通过双荧光标记（FDA-PI）验证细胞活力，结果表明在正确条件下，约90%的感觉细胞在解离后可在体外维持数小时。对角质板和纤毛中肌动蛋白的检测表明，该技术在形态学研究中有多种潜在应用，有助于对哺乳动物前庭细胞生理学的研究。
