Mo L Y
Beijing Institute of Otorhinolaryngology.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 1993;28(3):131-3, 185.
Young guinea pigs (250-300 g) were rapidly decapitated and the temporal bones were immediately removed. Under dissecting microscope, the crista ampullaris was removed and incubated in Hank's balanced salt solution containing 0.125 mg/ml collagenase (Sigma IV) at 37 degrees C for 50-60 min. After mechanical dissociation, the isolated cells were transferred onto the coverslips coated with poly-lysine. The cells were left 10-15 min to settle down on the coverslips. Afterward, the collagenase containing solution was replaced by Hank's solution. Observations were made under converted phase-contrast microscope. The results were as follows: 1. Vestibular hair cells are classified into two types. Type I cell was flask-shaped with sensory hairs, a cuticular plate, a long neck and a round bottom containing a large nucleus. The length of type I cell was 18.10 +/- 2.05 microns (mean +/- s n = 32). Type II cells were round shaped or cylindrically shaped without neck but with sensory hairs, a cuticular plate. The longitudinal axis of cylindrically shaped cell was 14.00 +/- 3.16 microns and horizontal axis 9.88 +/- 2.01 microns (mean +/- s n = 29). The diameter of the round cell was 10.31 +/- 1.98 microns (mean +/- s n = 10). 2. The proportion of type I and type II cells was 61/39. 3. An average of 500-700 vestibular hair cells were obtained from each ampulla. 4. Generally, vestibular hair cells could live in Hank's solution for 7-8 hours. The earliest sign of viability degression was the appearance of granules in the cytoplasm.
选用体重250 - 300克的幼年豚鼠，迅速断头并立即取出颞骨。在解剖显微镜下，取下壶腹嵴并置于含有0.125毫克/毫升胶原酶（Sigma IV型）的汉克平衡盐溶液中，于37℃孵育50 - 60分钟。经机械解离后，将分离出的细胞转移至涂有聚赖氨酸的盖玻片上。让细胞在盖玻片上静置10 - 15分钟使其沉降。之后，用汉克溶液替换含胶原酶的溶液。在倒置相差显微镜下进行观察。结果如下：1. 前庭毛细胞分为两种类型。I型细胞呈烧瓶状，有感觉毛、角质板、长颈和含大细胞核的圆形底部。I型细胞长度为18.10±2.05微米（平均值±标准差，n = 32）。II型细胞呈圆形或圆柱形，无颈部，但有感觉毛和角质板。圆柱形细胞的纵轴为14.00±3.16微米，横轴为9.88±2.01微米（平均值±标准差，n = 29）。圆形细胞的直径为10.31±1.98微米（平均值±标准差，n = 10）。2. I型和II型细胞的比例为61/39。3. 每个壶腹平均获得500 - 700个前庭毛细胞。4. 一般来说，前庭毛细胞可在汉克溶液中存活7 - 8小时。活力下降的最早迹象是细胞质中出现颗粒。
