Takumida M, Miyawaki H, Arishige S, Harada Y
Department of Otolaryngology, Hiroshima University School of Medicine, Japan.
ORL J Otorhinolaryngol Relat Spec. 1994 Jul-Aug;56(4):190-4. doi: 10.1159/000276654.
Vestibular sensory cells were isolated from the utricular macula or cristae ampullares of the guinea pig by enzymatic and mechanical dissociation. The isolated cells were classified into three types: flask-shaped type I sensory cells, rod-shaped type II sensory cells and round-shaped supporting cells. The cilia of type I sensory cells in the crista ampullaris were longer than those in the corresponding cell type in the utricular macula, while no morphological differences of the cell bodies were noted between crista ampullaris and utricular macula. Isolated living vestibular cells have a motile capacity. After exposure to a hypo-osmotic medium, the type I sensory cells showed tilting of the hair bundle. This change in shape may be closely related to the active mechanical transduction control.
通过酶解和机械分离从豚鼠的椭圆囊斑或壶腹嵴中分离出前庭感觉细胞。分离出的细胞分为三种类型：烧瓶状的I型感觉细胞、杆状的II型感觉细胞和圆形的支持细胞。壶腹嵴中I型感觉细胞的纤毛比椭圆囊斑中相应细胞类型的纤毛更长，而壶腹嵴和椭圆囊斑之间的细胞体在形态上没有差异。分离出的活前庭细胞具有运动能力。暴露于低渗介质后，I型感觉细胞的毛束出现倾斜。这种形状变化可能与主动机械转导控制密切相关。
