Ohtani M, Yamashita T, Amano H, Harada N, Kumazawa T
Department of Otolaryngology, Kansai Medical University, Osaka, Japan.
ORL J Otorhinolaryngol Relat Spec. 1991;53(2):82-5. doi: 10.1159/000276193.
Vestibular hair cells were isolated from the macula utriculi, macula sacculi and crista ampullaris of the guinea pig, using enzymatic and mechanical techniques. The cells could be classified into two types: flask-shaped ones with a neck and rod- or round-shaped ones without a neck. The flask-shaped cells were thought to be type I cells, the rod-shaped cells type II cells, while the round-shaped cells could have been type I or type II cells. The intracellular free calcium ion concentrations [( Ca2+]i) of these cells were determined using the Ca2(+)-sensitive dye Fura-2 and digital imaging miroscopy. In the resting state, [Ca2+]i in the nucleus and cuticular plate was variable in both types of cells. The 150 mM KCl stimulation, which might induce a depolarization, resulted in a temporary increase in [Ca2+]i in both types of cells. In the presence of 1 micron ionomycin, there was an irreversible increase in [Ca2+]i in both types of cells. These observations aid in elucidating vestibular function.
采用酶解和机械技术从豚鼠椭圆囊斑、球囊斑和壶腹嵴分离出前庭毛细胞。这些细胞可分为两类：有颈的烧瓶状细胞和无颈的杆状或圆形细胞。烧瓶状细胞被认为是I型细胞，杆状细胞是II型细胞，而圆形细胞可能是I型或II型细胞。使用钙敏感染料Fura-2和数字成像显微镜测定这些细胞的细胞内游离钙离子浓度[(Ca2+]i)。在静息状态下，两类细胞的细胞核和表皮板中的[Ca2+]i均有变化。150 mM KCl刺激可能会诱导去极化，导致两类细胞中的[Ca2+]i暂时升高。在存在1微摩尔离子霉素的情况下，两类细胞中的[Ca2+]i均出现不可逆升高。这些观察结果有助于阐明前庭功能。
