Quan J X, Zhang Y P, Han J L, Men Z M
Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Science, China.
Yi Chuan Xue Bao. 2000;27(5):383-90.
In this paper the mitochondrial DNA (mtDNA) of 87 domestic camels from Gansu, Xingjiang and Inner Mongolia were analyzed. Totally 18 restriction endonucleases including Apa I, Ava I, BamH I, Bcl I, Bgl I, Bgl II, Dra I, EcoR I, EcoR V, Hind III, Kpn I, Pvu II, Sac I, Sal I, Sca I, Sma I, Xba I and Xho I were used to investigate the restriction fragment length polymorphism (RFLP) of camel mtDNA through Southern blotting to reveal the genetic diversity within three groups of domestic camel and the genetic relationships among the three domestic groups. The results indicated that the average molecular weight of camel mtDNA is 16.26 +/- 0.28 kb, the camel mtDNA is inherited maternally and no variations and heteroplasmy are found in camel mtDNA. Among the 82 domestic camels, 35 restriction morph and 12 haplotypes were detected, and the value of average sequence variation within the domestic population (pi) is 0.2273%, which showed a relatively rich genetic diversity existed in the domestic camels. From F-test of the haplotype distribution and comparison of the haplotype sharement between the 3 domestic camel groups, it was suggested that there is no obvious genetic divergence and that the rich genetic diversity comes from the variation among individuals instead of the genetic divergence between groups. All domestic camels could be divided into 2 types (A and B) with a significant genetic distance (P = 0.0464).
本文对来自甘肃、新疆和内蒙古的87头家养骆驼的线粒体DNA（mtDNA）进行了分析。使用包括Apa I、Ava I、BamH I、Bcl I、Bgl I、Bgl II、Dra I、EcoR I、EcoR V、Hind III、Kpn I、Pvu II、Sac I、Sal I、Sca I、Sma I、Xba I和Xho I在内的18种限制性内切酶，通过Southern杂交研究骆驼mtDNA的限制性片段长度多态性（RFLP），以揭示三组家养骆驼群体内部的遗传多样性以及这三个家养群体之间的遗传关系。结果表明，骆驼mtDNA的平均分子量为16.26±0.28 kb，骆驼mtDNA呈母系遗传，且在骆驼mtDNA中未发现变异和异质性。在82头家养骆驼中，检测到35种限制性形态和12种单倍型，家养群体内的平均序列变异值（pi）为0.2273%，表明家养骆驼存在较为丰富的遗传多样性。通过对单倍型分布的F检验以及对三个家养骆驼群体之间单倍型共享情况的比较，表明不存在明显的遗传分化，丰富的遗传多样性来自个体间的变异而非群体间的遗传分化。所有家养骆驼可分为2种类型（A和B），遗传距离显著（P = 0.0464）。
