Simezo Ana Paula, da Silveira Bueno Carlos Eduardo, Cunha Rodrigo Sanches, Pelegrine Rina Andrea, Rocha Daniel Guimarães Pedro, de Martin Alexandre Sigrist, Kato Augusto Shoji
Department of Endodontics, São Leopoldo Mandic Dental Research Center, Campinas, São Paulo, Brazil.
Division of Endodontics, University of Manitoba, Winnipeg, Manitoba, Canada.
J Endod. 2017 Jan;43(1):141-146. doi: 10.1016/j.joen.2016.09.016. Epub 2016 Dec 6.
The aim of this study was to assess ex vivo the erosive effects of passive ultrasonic irrigation versus irrigation with reciprocating activation on the dentinal surface of the root canal at 3 predetermined levels using environmental scanning electron microscopy.
Ten roots of mandibular premolars were prepared using the ProTaper Universal system (Dentsply Maillefer, Ballaigues, Switzerland). The specimens were embedded in flasks cleaved longitudinally, and indentations were made 3.0, 6.0, and 9.0 mm from the apex. The specimens in the control group (n = 10) were cleaned in an ultrasonic bath containing 2.5% sodium hypochlorite and 17% EDTA and then dried. Then, environmental scanning electron microscopic images were obtained at magnification × 800. The specimens were then reassembled in their flasks, and the NaOCl and EDTA solutions were activated according to the conditions established for the experimental groups (ie, the passive ultrasonic irrigation group [n = 5] and the EasyClean (Easy Equipamentos Odontológicos, Belo Horizonte, MG, Brazil) group, irrigation with reciprocating activation with the EasyClean instrument [n = 5]). The specimens of both experimental groups were analyzed in the same manner as in the control group. Analysis of the dentinal surface topography was conducted using the 3D Roughness Reconstruction program (Phenom-World BV, Eindhoven, the Netherlands) as a means for assessing erosion. The data were evaluated by means of the Kruskal-Wallis, Student-Newman-Keuls, and Mann-Whitney tests.
In the EasyClean group, the degree of dentinal erosion at 3.0 mm was significantly higher than at 9.0 mm. In the other comparisons, there was no statistically significant difference (P < .05).
The final irrigation techniques tested were equivalent in relation to the degree of erosion caused to the dentinal surface.
本研究的目的是使用环境扫描电子显微镜，在3个预定水平上体外评估被动超声冲洗与往复式激活冲洗对根管牙本质表面的侵蚀作用。
使用ProTaper Universal系统（登士柏迈丽菲，瑞士巴拉格）预备10颗下颌前磨牙的牙根。将标本纵向劈开后嵌入烧瓶中，并在距根尖3.0、6.0和9.0毫米处制作压痕。对照组（n = 10）的标本在含有2.5%次氯酸钠和17%乙二胺四乙酸的超声浴中清洗，然后干燥。然后，以800倍放大倍数获取环境扫描电子显微镜图像。然后将标本重新组装到烧瓶中，并根据为实验组设定的条件激活次氯酸钠和乙二胺四乙酸溶液（即被动超声冲洗组[n = 5]和EasyClean组[巴西米纳斯吉拉斯州贝洛奥里藏特市Easy Equipamentos Odontológicos公司]，使用EasyClean器械进行往复式激活冲洗[n = 5]）。两个实验组的标本均按照与对照组相同的方式进行分析。使用3D粗糙度重建程序（荷兰埃因霍温市Phenom-World BV公司）对牙本质表面形貌进行分析，作为评估侵蚀的一种手段。数据通过Kruskal-Wallis检验、Student-Newman-Keuls检验和Mann-Whitney检验进行评估。
在EasyClean组中，3.0毫米处的牙本质侵蚀程度显著高于9.0毫米处。在其他比较中，没有统计学上的显著差异（P <.05）。
所测试的最终冲洗技术在对牙本质表面造成的侵蚀程度方面是等效的。
