Oliveira Adriana, Rocha Daniel, De Martin Alexandre, Limoeiro Ana-Grasiela, Nascimento Wayne, Fontana Carlos, Pelegrine Rina, Martinez Elizabeth, Bueno Carlos
Department of Endodontics, Faculdade de Ilhéus Bahia, Brazil.
J Clin Exp Dent. 2022 Aug 1;14(8):e646-e651. doi: 10.4317/jced.59547. eCollection 2022 Aug.
To evaluate the antimicrobial activity of the following chelating agents against Enterococcus faecalis using quantitative polymerase chain reaction (qPCR) analysis: 1% peracetic acid (PA), 1% peracetic acid with 0.1% cetrimide (PAC), and 17% ethylenediaminetetraacetic acid (EDTA) activated by passive ultrasonic irrigation (PUI) or with Easy Clean (EC), all followed by 2.5% sodium hypochlorite (NaOCl).
A total of 80 permanent human mandibular premolars were randomly divided into eight experimental groups according to the chemical solution and agitation protocol used: Group PA + PUI; Group PA + EC; Group PAC + PUI; PAC + EC; group EDTA + PUI; EDTA + EC, all followed by 2.5% NaOCl; and two control groups with saline solution(NaCl): NaCl + PUI and NaCl + EC. Microbial samples were collected before (S1) and after the irrigation protocol (S2). Intracanal E. faecalis reduction analysis was performed by qPCR. Intragroup analysis was performed using the Wilcoxon signed-rank test for paired data, and intergroup analysis was performed using the Wilcoxon Mann-Whitney U test for independent samples. The significance level was set at < 0.05.
A significant difference was found between S1 and S2 in all groups except NaCl+ EC ( = 0.1602). Comparison between groups showed that PAC + PUI was significantly different from PA +EC ( = 0.0448).
The activated chelating agents were effective against , with significant results compared to the control groups. The peracetic acid with cetrimide activated by PUI showed better results than peracetic acid with EC. Easy clean, Final irrigation protocols, Passive ultrasonic irrigation, Peracetic acid.
使用定量聚合酶链反应（qPCR）分析评估以下螯合剂对粪肠球菌的抗菌活性：1%过氧乙酸（PA）、含0.1%西曲溴铵的1%过氧乙酸（PAC）以及通过被动超声冲洗（PUI）或使用易清洁（EC）激活的17%乙二胺四乙酸（EDTA），所有处理后均使用2.5%次氯酸钠（NaOCl）。
根据所使用的化学溶液和冲洗方案，将总共80颗人类永久性下颌前磨牙随机分为八个实验组：PA + PUI组；PA + EC组；PAC + PUI组；PAC + EC组；EDTA + PUI组；EDTA + EC组，所有组处理后均使用2.5% NaOCl；以及两个使用盐溶液（NaCl）的对照组：NaCl + PUI组和NaCl + EC组。在冲洗方案实施前（S1）和实施后（S2）收集微生物样本。通过qPCR进行根管内粪肠球菌减少分析。组内分析使用配对数据的Wilcoxon符号秩检验，组间分析使用独立样本的Wilcoxon Mann - Whitney U检验。显著性水平设定为<0.05。
除NaCl + EC组（P = 0.1602）外，所有组的S1和S2之间均存在显著差异。组间比较显示，PAC + PUI组与PA + EC组有显著差异（P = 0.0448）。
激活的螯合剂对粪肠球菌有效，与对照组相比有显著效果。通过PUI激活的含西曲溴铵过氧乙酸比含EC的过氧乙酸效果更好。易清洁；最终冲洗方案；被动超声冲洗；过氧乙酸
