de Freitas Rafaela Pignatti, Greatti Vanessa Raquel, Alcalde Murilo Priori, Cavenago Bruno Cavalini, Vivan Rodrigo Ricci, Duarte Marco Antonio Hungaro, Weckwerth Ana Carolina Villas Bôas, Weckwerth Paulo Henrique
Universidade do Sagrado Coração, Pro-Rectory of Research and Post Graduation, Bauru, Sao Paulo, Brazil.
Department of Drugs and Medical, Pharmaceutical Biotechnology Laboratory, School of Pharmaceutical Sciences, São Paulo State University, Araraquara, Sao Paulo, Brazil.
J Endod. 2017 Jan;43(1):131-134. doi: 10.1016/j.joen.2016.09.014. Epub 2016 Dec 6.
The objective of the present study was to evaluate the in vitro antibiofilm activity and pH of calcium hydroxide associated with different nonsteroidal anti-inflammatory drugs (NSAIDs).
The groups analyzed were as follows: group 1, calcium hydroxide paste with propylene glycol; group 2, calcium hydroxide paste with propylene glycol + 5% diclofenac sodium; group 3, calcium hydroxide paste with propylene glycol + 5% ibuprofen; group 4, calcium hydroxide paste with propylene glycol + 5% ciprofloxacin; and group 6, positive control (without medication). For analysis of the pH, the pastes were inserted into tubes and immersed in flasks containing ultrapure water. At the time intervals of 3, 24, 72, and 168 hours, the pH was measured with a calibrated pH meter. For microbial analysis, biofilm was induced in 30 bovine dentin blocks for 21 days. Subsequently, the pastes were placed on the blocks with biofilm for 7 days. Afterward, the pastes were removed by irrigation with sterile water, and the specimens were analyzed with a laser scanning confocal microscope with the 50 μL Live/Dead BacLight Bacterial Viability solution L7012 Kit (Molecular Probes, Inc, Eugene, OR). Data were subjected to statistical analysis at a significance level of 5%.
The highest pH values were found for calcium hydroxide associated with ciprofloxacin in all periods analyzed. With the exception of pure calcium hydroxide paste, the other groups showed statistically significant differences (P < .05) in comparison with the positive control.
The association of NSAIDs or antibiotic did not interfere with the pH of calcium hydroxide paste and increased the antimicrobial action of calcium hydroxide paste against Enterococcus faecalis biofilm formation.
本研究的目的是评估与不同非甾体抗炎药（NSAIDs）联合使用时氢氧化钙的体外抗生物膜活性和pH值。
分析的组别如下：第1组，含丙二醇的氢氧化钙糊剂；第2组，含丙二醇+5%双氯芬酸钠的氢氧化钙糊剂；第3组，含丙二醇+5%布洛芬的氢氧化钙糊剂；第4组，含丙二醇+5%环丙沙星的氢氧化钙糊剂；第6组，阳性对照（无药物）。为分析pH值，将糊剂插入试管并浸入装有超纯水的烧瓶中。在3、24、72和168小时的时间间隔，用校准后的pH计测量pH值。对于微生物分析，在30个牛牙本质块中诱导生物膜形成21天。随后，将糊剂置于有生物膜的块上7天。之后，用无菌水冲洗去除糊剂，并用配备50μL Live/Dead BacLight细菌活力溶液L7012试剂盒（Molecular Probes公司，俄勒冈州尤金市）的激光扫描共聚焦显微镜对标本进行分析。数据在5%的显著性水平下进行统计分析。
在所有分析时间段内，与环丙沙星联合使用的氢氧化钙的pH值最高。除纯氢氧化钙糊剂外，其他组与阳性对照相比均有统计学显著差异（P<0.05）。
NSAIDs或抗生素的联合使用不会干扰氢氧化钙糊剂的pH值，并增强了氢氧化钙糊剂对粪肠球菌生物膜形成的抗菌作用。
