Ferrer-Luque Carmen María, Solana Carmen, Aguado Beatriz, Ruiz-Linares Matilde
Department of Stomatology, University of Granada, 18071 Granada, Spain.
Instituto de Investigación Biosanitaria ibs.GRANADA, 18012 Granada, Spain.
Antibiotics (Basel). 2023 Feb 23;12(3):450. doi: 10.3390/antibiotics12030450.
Persistent infections have become a challenge in dentistry because of growing antibiotic resistance. Nonsteroidal anti-inflammatory drugs (NSAIDs) appear to be a therapeutic alternative to control biofilm infection. The objective of this work is to evaluate the antimicrobial activity and cytotoxicity of sodium diclofenac (DCS), ibuprofen (IBP) and ibuprofen arginine (IBP-arginine) solutions against endodontic polymicrobial biofilms. Sterile radicular dentin blocks of 4 mm × 4 mm × 0.7 mm were used as substrate to grow biofilm. The dentin blocks were submerged into solutions for 5 min. The antimicrobial activity was evaluated by means of the adenosine triphosphate (ATP) assay and confocal laser scanning microscopy (CLSM). Fibroblasts 3T3-L1 (ECACC 86052701) were used to test the cytotoxicity of irrigating solutions. The antibiofilm effects determined by the ATP assay showed that 4% IBP-arginine solution exerted the highest antibiofilm activity, followed by 4% DCS and 4% IBP, with statistical differences among groups ( < 0.001). As for CLSM, 4% DCS and 4% IBP-arginine solutions gave the lowest viable cell percentages, without significant differences between them. Cytotoxicity results at 1/10 dilution were similar for all solutions. At 1/100 dilution, a 4% DCS solution obtained the lowest cell viability for both time periods assayed, 1 h and 24 h. The IBP-arginine group showed the highest cell viability at 24 h. In this preliminary study, in terms of antibiofilm activity and cytotoxicity, a mixed 4% IBP-arginine solution gave the most promising results. NSAID solutions could be recommendable drugs for endodontic disinfection procedures.
由于抗生素耐药性不断增加，持续性感染已成为牙科领域的一项挑战。非甾体抗炎药（NSAIDs）似乎是控制生物膜感染的一种治疗选择。这项工作的目的是评估双氯芬酸钠（DCS）、布洛芬（IBP）和布洛芬精氨酸（IBP-精氨酸）溶液对牙髓多微生物生物膜的抗菌活性和细胞毒性。使用4mm×4mm×0.7mm的无菌牙根牙本质块作为生物膜生长的底物。将牙本质块浸入溶液中5分钟。通过三磷酸腺苷（ATP）测定和共聚焦激光扫描显微镜（CLSM）评估抗菌活性。使用成纤维细胞3T3-L1（ECACC 86052701）测试冲洗液的细胞毒性。ATP测定确定的抗生物膜作用表明，4%的IBP-精氨酸溶液具有最高的抗生物膜活性，其次是4%的DCS和4%的IBP，各组之间存在统计学差异（<0.001）。至于CLSM，4%的DCS和4%的IBP-精氨酸溶液的活细胞百分比最低，两者之间无显著差异。所有溶液在1/10稀释时的细胞毒性结果相似。在1/100稀释时，4%的DCS溶液在1小时和24小时这两个检测时间段内的细胞活力最低。IBP-精氨酸组在24小时时的细胞活力最高。在这项初步研究中，就抗生物膜活性和细胞毒性而言，4%的IBP-精氨酸混合溶液给出了最有前景的结果。NSAID溶液可能是牙髓消毒程序中值得推荐的药物。
