Meireles André L F, Marques Marília R, Segabinazi Ethiane, Spindler Christiano, Piazza Francele V, Salvalaggio Gabriela S, Augustin Otávio A, Achaval Matilde, Marcuzzo Simone
Programa de Pós-graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; Laboratório de Histofisiologia Comparada, Departamento de Ciências Morfológicas, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Laboratório de Histofisiologia Comparada, Departamento de Ciências Morfológicas, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Brain Res Bull. 2017 Jan;128:58-67. doi: 10.1016/j.brainresbull.2016.12.001. Epub 2016 Dec 7.
Several physiotherapy approaches are used with different aims in the treatment of cerebral palsy (CP), such as the early stimulation and the locomotor training, but their biological effects, isolated or combined, are not completely known. In animals models, these strategies can be compared, with due translational restrictions, to the environmental enrichment (EE), that involves the enhancement of animal's physical and social environment, and locomotor stimulation (LS), that can be performed using the treadmill adapted for rats. This study was designed to describe which biological and functional mechanisms underlying rehabilitative process in clinical practice. Male rat pups were initially divided in two groups: control (healthy) and submitted to a CP model. Then, pups were divided in eight groups: CP, CPEE, CPLS, CPEELS and its respectively control groups. Functional outcomes were assessed at the postnatal day (P) 31 and P52. The tibialis anterior and soleus muscles, tibia bone parameters, the expression of synaptophysin in the primary motor cortex (M1) and ventral horn (VH) of the spinal cord, were evaluated. The association of therapies was able to improve the functional assessments and musculoskeletal parameters. Isolated therapies presented complementary benefits in CP, but the association of therapies proved to be a fundamental and effective strategy to functional recovery, besides alter positively all biological tissues evaluated in this study.
在脑瘫（CP）治疗中，有几种物理治疗方法被用于不同目的，如早期刺激和运动训练，但它们单独或联合使用的生物学效应尚未完全明确。在动物模型中，在适当的转化限制条件下，这些策略可与环境丰富化（EE）相比较，环境丰富化涉及增强动物的物理和社会环境，以及运动刺激（LS），运动刺激可使用适合大鼠的跑步机来进行。本研究旨在描述临床实践中康复过程背后的生物学和功能机制。雄性幼鼠最初被分为两组：对照组（健康组）和接受脑瘫模型制作的组。然后，幼鼠被分为八组：CP组、CPEE组、CPLS组、CPEELS组及其相应的对照组。在出生后第31天和第52天评估功能结果。评估了胫前肌和比目鱼肌、胫骨骨参数、脊髓初级运动皮层（M1）和腹角（VH）中突触素的表达。联合治疗能够改善功能评估和肌肉骨骼参数。单独的治疗在脑瘫中有互补的益处，但联合治疗被证明是功能恢复的一种基本且有效的策略，此外还能积极改变本研究中评估的所有生物组织。
