Chatterjee A, Saha D, Niemann H, Gryshkov O, Glasmacher B, Hofmann N
Institute for Multiphase Processes, Leibniz Universität Hannover, Hannover, Germany.
Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Mariensee, Neustadt, Germany.
Cryobiology. 2017 Feb;74:1-7. doi: 10.1016/j.cryobiol.2016.12.002. Epub 2016 Dec 9.
Effective cryopreservation protocols are essential for long-term storage of cells and their subsequent clinical application. Freezing protocols are generally considered as safe; however, putative effects on epigenetic marks have not yet been studied in detail. While post-thaw cell survival rates have been used to evaluate the success of cryopreservation protocols, increasing evidence suggests that freezing may be associated with deviations from the physiological epigenetic marks with putative long-term effects on the cells and/or their derivatives. A better understanding of the underlying mechanisms would be beneficial for improving safety and effectiveness of freezing protocols. The purpose of this review is to provide current information regarding epigenetic alterations (DNA methylation and histone modification patterns) associated with cryopreservation.
有效的冷冻保存方案对于细胞的长期储存及其后续临床应用至关重要。冷冻方案通常被认为是安全的；然而，其对表观遗传标记的潜在影响尚未得到详细研究。虽然解冻后细胞存活率已被用于评估冷冻保存方案的成功与否，但越来越多的证据表明，冷冻可能与生理表观遗传标记的偏差有关，对细胞和/或其衍生物具有潜在的长期影响。更好地理解潜在机制将有助于提高冷冻方案的安全性和有效性。本综述的目的是提供有关与冷冻保存相关的表观遗传改变（DNA甲基化和组蛋白修饰模式）的当前信息。
