Wang Mei, Wang Yan-Hong, Avula Bharathi, Radwan Mohamed M, Wanas Amira S, Mehmedic Zlatko, van Antwerp John, ElSohly Mahmoud A, Khan Ikhlas A
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA.
Department of Pharmacognosy, Faculty of Pharmacy, University of Alexandria, Alexandria, Egypt.
J Forensic Sci. 2017 May;62(3):602-611. doi: 10.1111/1556-4029.13341. Epub 2016 Dec 13.
Ultra-high-performance supercritical fluid chromatography (UHPSFC) is an efficient analytical technique and has not been fully employed for the analysis of cannabis. Here, a novel method was developed for the analysis of 30 cannabis plant extracts and preparations using UHPSFC/PDA-MS. Nine of the most abundant cannabinoids, viz. CBD, ∆ -THC, THCV, ∆ -THC, CBN, CBG, THCA-A, CBDA, and CBGA, were quantitatively determined (RSDs < 6.9%). Unlike GC methods, no derivatization or decarboxylation was required prior to UHPSFC analysis. The UHPSFC chromatographic separation of cannabinoids displayed an inverse elution order compared to UHPLC. Combining with PDA-MS, this orthogonality is valuable for discrimination of cannabinoids in complex matrices. The developed method was validated, and the quantification results were compared with a standard UHPLC method. The RSDs of these two methods were within ±13.0%. Finally, chemometric analysis including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to differentiate between cannabis samples.
超高效超临界流体色谱法（UHPSFC）是一种高效的分析技术，尚未完全用于大麻分析。在此，开发了一种使用UHPSFC/PDA-MS分析30种大麻植物提取物和制剂的新方法。对九种含量最高的大麻素，即CBD、∆-THC、THCV、∆-THC、CBN、CBG、THCA-A、CBDA和CBGA进行了定量测定（相对标准偏差<6.9%）。与气相色谱法不同，UHPSFC分析前无需衍生化或脱羧。与超高效液相色谱法相比，大麻素的UHPSFC色谱分离呈现出相反的洗脱顺序。结合PDA-MS，这种正交性对于复杂基质中大麻素的鉴别很有价值。对所开发的方法进行了验证，并将定量结果与标准超高效液相色谱法进行了比较。这两种方法的相对标准偏差在±13.0%以内。最后，使用包括主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）在内的化学计量学分析来区分大麻样品。
