Toyota Kenji, Sato Tomomi, Tatarazako Norihisa, Iguchi Taisen
Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, Department of Basic Biology, Faculty of Life Science, SOKENDAI (Graduate University for Advanced Studies), 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan.
Environmental Genomics Group, School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Biol Open. 2017 Feb 15;6(2):161-164. doi: 10.1242/bio.021857.
Sex determination of is decided by environmental conditions. We established a suitable experimental system for this study using WTN6 strain, in which the sex of the offspring can be controlled by photoperiod. Long-day conditions induced females and short-day conditions induced males. Using this system, we previously found that methy farnesoate (MF), which is a putative innate juvenile hormone molecule in daphnids, is necessary for male sex determination and that protein kinase C (PKC) is a candidate factor of male sex determiner. In this study, we demonstrated that a PKC inhibitor [bisindolylmaleimide IV (BIM)] application strongly suppressed male offspring induction in the short-day condition. Moreover, co-treatment of BIM with MF revealed that PKC signaling acts upstream of MF signaling for male sex determination. This is the first experimental evidence that PKC is involved in the male sex determination process associated with methyl farnesoate signaling in daphnid species.
性别的决定取决于环境条件。我们使用WTN6菌株建立了一个适合本研究的实验系统，在该系统中，后代的性别可以通过光周期来控制。长日照条件诱导产生雌性，短日照条件诱导产生雄性。利用这个系统，我们之前发现，法尼醇甲酯（MF）是水蚤中一种假定的先天性保幼激素分子，是雄性性别决定所必需的，并且蛋白激酶C（PKC）是雄性性别决定因子的一个候选因素。在本研究中，我们证明了应用PKC抑制剂[双吲哚马来酰亚胺IV（BIM）]在短日照条件下强烈抑制了雄性后代的诱导。此外，BIM与MF的联合处理表明，PKC信号在雄性性别决定中作用于MF信号的上游。这是PKC参与水蚤物种中法尼醇甲酯信号相关的雄性性别决定过程的首个实验证据。
