Hegde Mithra N, Hegde Nishanth N, Shetty Preethesh, Hegde Nishmitha N, Vittal Chaithra Lakshmi, Ravinanthan Manikandan
Department of Conservative Dentistry and Endodontics, AB Shetty Memorial Institute of Dental Sciences, Nitte (Deemed to be University), Mangalore, Karnataka, India.
Department of Restorative Dental Sciences, Faculty of Dentistry, Al Baha University, Al Baha, Saudi Arabia.
J Conserv Dent Endod. 2024 Sep;27(9):983-987. doi: 10.4103/JCDE.JCDE_340_24. Epub 2024 Sep 7.
Dental caries, commonly known as tooth decay is a widespread oral health problem mainly attributed to the activity of cariogenic bacteria, such as and species. Tooth Mousse, containing remineralizing agents, herbal and fluoride containing toothpaste with antimicrobial agents have been developed to target cariogenic bacteria. Herbal, fluoride toothpaste, and Tooth Mousse are commonly prescribed to prevent, reduce, and control dental caries.
This study aims to analyze the effect of Tooth Mousse and medicated toothpastes on and using direct contact test.
and were cultured on Mueller-Hinton agar (MHA-Hi media) using sterile cotton swabs and plates were dried for 15 min. Toothpastes (Dabur Red, Pepsodent) and Tooth Mousse were used at 1:1 dilution using sterile pyrogen-free distilled water. Fifty microliter of toothpastes and Tooth Mousse were introduced into each well. The plates were incubated at 37°C for 24 h.
The antimicrobial activity was evaluated by measuring the diameter of zones of inhibition (mm). The toothpaste containing fluoride (A) showed greater zone of inhibition compared to herbal toothpaste (B) whereas Tooth Mousse (C) did not show any zone of inhibition.
Among herbal and fluoride toothpaste, fluoride containing toothpaste showed more zone of inhibition thereby attributing to its increased antimicrobial property on S. mutans and L. acidophilus.
龋齿，通常被称为蛀牙，是一种广泛存在的口腔健康问题，主要归因于致龋菌的活动，如变形链球菌和嗜酸乳杆菌。含再矿化剂的齿膜、含草药和抗菌剂的含氟牙膏已被开发用于针对致龋菌。草药牙膏、含氟牙膏和齿膜通常被开出处方用于预防、减少和控制龋齿。
本研究旨在通过直接接触试验分析齿膜和药用牙膏对变形链球菌和嗜酸乳杆菌的影响。
使用无菌棉签在穆勒-欣顿琼脂（MHA-Hi培养基）上培养变形链球菌和嗜酸乳杆菌，平板干燥15分钟。牙膏（达布尔红牙膏、佳洁士牙膏）和齿膜用无菌无热原蒸馏水按1:1稀释。将50微升牙膏和齿膜分别加入每个孔中。平板在37°C下孵育24小时。
通过测量抑菌圈直径（毫米）评估抗菌活性。含氟牙膏（A）的抑菌圈比草药牙膏（B）更大，而齿膜（C）没有显示出任何抑菌圈。
在草药牙膏和含氟牙膏中，含氟牙膏显示出更大的抑菌圈，因此其对变形链球菌和嗜酸乳杆菌的抗菌性能增强。
