Wang Guan H, Sun Bao F, Xiong Tuan L, Wang Yan K, Murfin Kristen E, Xiao Jin H, Huang Da W
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of SciencesBeijing, China; University of Chinese Academy of SciencesBeijing, China.
Disease Genomics and Individualized Medicine Laboratory, Beijing Institute of Genomics, Chinese Academy of Sciences Beijing, China.
Front Microbiol. 2016 Nov 29;7:1867. doi: 10.3389/fmicb.2016.01867. eCollection 2016.
Phage-mediated horizontal gene transfer (HGT) is common in free-living bacteria, and many transferred genes can play a significant role in their new bacterial hosts. However, there are few reports concerning phage-mediated HGT in endosymbionts (obligate intracellular bacteria within animal or plant hosts), such as . The -infecting temperate phage WO can actively shift among genomes and has the potential to mediate HGT between strains. In the present study, we extend previous findings by validating that the phage WO can mediate transfer of non-phage genes. To do so, we utilized bioinformatic, phylogenetic, and molecular analyses based on all sequenced and phage WO genomes. Our results show that the phage WO can mediate HGT between strains, regardless of whether the transferred genes originate from or other unrelated bacteria.
噬菌体介导的水平基因转移（HGT）在自由生活的细菌中很常见，许多转移的基因可以在它们新的细菌宿主中发挥重要作用。然而，关于噬菌体介导的HGT在共生菌（动植物宿主内的专性细胞内细菌）中的报道很少，比如。感染的温和噬菌体WO可以在基因组之间活跃转移，并有潜力介导菌株之间的HGT。在本研究中，我们通过验证噬菌体WO可以介导非噬菌体基因的转移来扩展先前的发现。为此，我们基于所有已测序的和噬菌体WO基因组进行了生物信息学、系统发育和分子分析。我们的结果表明，噬菌体WO可以介导菌株之间的HGT，无论转移的基因是来自还是其他不相关的细菌。
