Lis Lukas, Connerton Ian F
PTC Phage Technology Center GmbH, Im Kompetenzzentrum BioSecurityBönen, Germany; Division of Food Sciences, School of Biosciences, University of NottinghamLoughborough, UK.
Division of Food Sciences, School of Biosciences, University of Nottingham Loughborough, UK.
Front Microbiol. 2016 Nov 29;7:1908. doi: 10.3389/fmicb.2016.01908. eCollection 2016.
A screen of bacteriophages infecting a panel of PT14 gene knock-out mutants identified a role for the minor flagellin encoded by the gene, in the defense of the host against CP8unalikevirus bacteriophage CP_F1 infection. Inactivation of the gene resulted in an increase in the susceptibility of PT14 cultures to infection by CP_F1 and an increase in bacteriophage yields. Infection of wild type PT14 with CP_F1 produces turbid plaques in bacterial lawns, from which 78% of the resistant isolates recovered exhibit either attenuation or complete loss of motility. CP_F1 produces clear plaques on the mutant with no regrowth in the lysis zones. Complementation of the mutant restored overgrowth and the development of resistance at the expense of motility. Further analyses revealed an increase in bacteriophage adsorption constant of nearly 2-fold and burst-size 3-fold, relative to the wild type. Motility analysis showed no major reduction in swarming motility in the mutant. Thus, we propose a new role for FlaB in the defense of campylobacters against bacteriophage infection.
对感染一组PT14基因敲除突变体的噬菌体进行筛选，确定了该基因编码的次要鞭毛蛋白在宿主抵御CP8类病毒噬菌体CP_F1感染中的作用。该基因的失活导致PT14培养物对CP_F1感染的易感性增加以及噬菌体产量增加。用CP_F1感染野生型PT14会在细菌菌苔中产生浑浊噬菌斑，从这些噬菌斑中回收的78%抗性分离株表现出运动性减弱或完全丧失。CP_F1在该突变体上产生清晰噬菌斑，裂解区域无再生长。突变体的互补恢复了过度生长和抗性的发展，但以运动性为代价。进一步分析表明，相对于野生型，噬菌体吸附常数增加了近2倍，裂解量增加了3倍。运动性分析表明该突变体的群体运动性没有大幅降低。因此，我们提出FlaB在弯曲杆菌抵御噬菌体感染中具有新作用。
