Corcionivoschi Nicolae, Balta Igori, Butucel Eugenia, McCleery David, Pet Ioan, Iamandei Maria, Stef Lavinia, Morariu Sorin
Bacteriology Branch, Veterinary Sciences Division, Agri-Food and Biosciences Institute, Belfast BT4 3SD, UK.
Faculty of Bioengineering of Animal Resources, University of Life Sciences King Mihai I from Timisoara, 300645 Timisoara, Romania.
Foods. 2023 Oct 21;12(20):3863. doi: 10.3390/foods12203863.
The contact and adherence of bacteria to various surfaces has significant consequences on biofilm formation through changes in bacterial surface structures or gene expression with potential ramifications on plant and animal health. Therefore, this study aimed to investigate the effect of organic acid-based mixtures (Ac) on the ability and to attach and form biofilm on various surfaces, including plastic, chicken carcass skins, straw bedding, and eggshells. Moreover, we aimed to explore the effect of Ac on the expression of (S, C, D) and (S, A, B) bacterial genes involved in the attachment and biofilm formation via changes in bacterial surface polysaccharidic structures. Our results show that Ac had a significant effect on the expression of these genes in bacteria either attached to these surfaces or in planktonic cells. Moreover, the significant decrease in bacterial adhesion was coupled with structural changes in bacterial surface polysaccharide profiles, impacting their adhesion and biofilm-forming ability. Essentially, our findings accentuate the potential of natural antimicrobials, such as Ac, in reducing bacterial attachment and biofilm formation across various environments, suggesting promising potential applications in sectors like poultry production and healthcare.
细菌与各种表面的接触和黏附通过细菌表面结构或基因表达的变化对生物膜形成产生重大影响，这可能会对动植物健康产生潜在影响。因此，本研究旨在探究基于有机酸的混合物（Ac）对细菌在包括塑料、鸡胴体皮肤、稻草垫料和蛋壳等各种表面上附着和形成生物膜能力的影响。此外，我们旨在通过细菌表面多糖结构的变化来探索Ac对参与附着和生物膜形成的（S、C、D）和（S、A、B）细菌基因表达的影响。我们的结果表明，Ac对附着在这些表面上的细菌或浮游细胞中这些基因的表达有显著影响。此外，细菌黏附的显著降低与细菌表面多糖谱的结构变化相关，影响了它们的黏附及生物膜形成能力。从本质上讲，我们的研究结果凸显了天然抗菌剂（如Ac）在减少各种环境中细菌附着和生物膜形成方面的潜力，这表明其在家禽生产和医疗保健等领域具有广阔的潜在应用前景。
