Bodanapu Reddaiah, Gupta Suresh K, Basha Pinjari O, Sakthivel Kannabiran, Sreelakshmi Yellamaraju, Sharma Rameshwar
Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad Hyderabad, India.
Front Plant Sci. 2016 Nov 28;7:1714. doi: 10.3389/fpls.2016.01714. eCollection 2016.
Nitric oxide (NO) plays a pivotal role in growth and disease resistance in plants. It also acts as a secondary messenger in signaling pathways for several plant hormones. Despite its clear role in regulating plant development, its role in fruit development is not known. In an earlier study, we described a () mutant of tomato, whose phenotype results from hyperaccumulation of NO. The molecular mapping localized locus in 2.5 Mb region of chromosome 9. The mutant showed sluggish growth, with smaller leaves, flowers and was less fertile than wild type. The mutant also showed reduced fruit size and slower ripening of the fruits post-mature green stage to the red ripe stage. Comparison of the metabolite profiles of fruits with wild-type fruits during ripening revealed a significant shift in the patterns. In fruits intermediates of the tricarboxylic acid (TCA) cycle were differentially regulated than WT indicating NO affected the regulation of TCA cycle. The accumulation of several amino acids, particularly tyrosine, was higher, whereas most fatty acids were downregulated in fruits. Among the plant hormones at one or more stages of ripening, ethylene, Indole-3-acetic acid and Indole-3-butyric acid increased in , whereas abscisic acid declined. Our analyses indicate that the retardation of fruit growth and ripening in mutant likely results from the influence of NO on central carbon metabolism and endogenous phytohormones levels.
一氧化氮（NO）在植物的生长和抗病性中起着关键作用。它还在几种植物激素的信号传导途径中作为第二信使发挥作用。尽管其在调节植物发育方面的作用明确，但其在果实发育中的作用尚不清楚。在早期的一项研究中，我们描述了一种番茄的（）突变体，其表型是由NO的过度积累导致的。分子定位将该基因座定位在9号染色体的2.5 Mb区域。该突变体生长迟缓，叶片、花朵较小，且育性低于野生型。该突变体还表现出果实大小减小，果实从成熟绿期到红熟期的成熟速度减慢。在成熟过程中对突变体果实和野生型果实的代谢物谱进行比较，发现模式有显著变化。在突变体果实中，三羧酸（TCA）循环的中间产物与野生型相比受到不同程度的调节，表明NO影响了TCA循环的调节。几种氨基酸，特别是酪氨酸的积累较高，而突变体果实中的大多数脂肪酸下调。在果实成熟的一个或多个阶段，乙烯、吲哚 - 3 - 乙酸和吲哚 - 3 - 丁酸在突变体中增加，而脱落酸下降。我们的分析表明，突变体果实生长和成熟的延迟可能是由于NO对中心碳代谢和内源植物激素水平的影响。
