Mangas Arturo, Yajeya Javier, Gonzalez Noelia, Husson Marianne, Geffard Michel, Coveñas Rafael
Gemacbio, Saint Jean d'Illac, France Institut pour le Développement de la Recherche en Pathologie Humaine et Thérapeutique (IDRPHT), Talence, France Institute of Neurosciences of Castilla y León (INCYL), Laboratory of Neuroanatomy of the Peptidergic Systems, University of Salamanca, Salamanca, Spain.
Folia Histochem Cytobiol. 2016;54(4):186-192. doi: 10.5603/FHC.a2016.0024. Epub 2016 Dec 14.
The available immunohistochemical techniques have documented restricted distribution of vitamins in the mammalian brain. The aim of the study was to develop a highly specific antiserum directed against pantothenic acid to explore the presence of this vitamin in the mammalian brain.
According to ELISA tests, the anti-pantothenic acid antiserum used showed a good affinity (10-8 M) and specificity. The antiserum was raised in rabbits. Using an indirect immunoperoxidase technique, the mapping of pantothenic acid-immunoreactive structures was carried out in the rat brain.
Pantothenic acid-immunoreactive perikarya were exclusively found in the intermediate part of the lateral septal nucleus. These cells were generally small, round, fusiform or pyramidal and showed 2-3 long (50-100 μm) immunoreactive dendrites. Any immunoreactive axons containing pantothenic acid were detected.
The very restricted anatomical distribution of the pantothenic acid suggests that this vitamin could be involved in some specific neurophysiological mechanisms.
现有的免疫组织化学技术已证明维生素在哺乳动物大脑中的分布有限。本研究的目的是开发一种针对泛酸的高特异性抗血清，以探索这种维生素在哺乳动物大脑中的存在情况。
根据酶联免疫吸附测定（ELISA）试验，所用的抗泛酸抗血清显示出良好的亲和力（10-8M）和特异性。该抗血清是在兔子体内产生的。使用间接免疫过氧化物酶技术，在大鼠脑中对泛酸免疫反应性结构进行定位。
泛酸免疫反应性核周体仅在外侧隔核的中间部分发现。这些细胞通常较小，呈圆形、梭形或锥形，并显示出2-3条长（50-100μm）的免疫反应性树突。未检测到任何含有泛酸的免疫反应性轴突。
泛酸非常有限的解剖学分布表明这种维生素可能参与某些特定的神经生理机制。
