Institute of Neurosciences of Castilla y León (INCYL), Laboratory 14, Salamanca, Spain.
Neurosci Lett. 2012 Feb 10;509(1):64-8. doi: 10.1016/j.neulet.2011.12.053. Epub 2012 Jan 3.
In order to increase our knowledge about the distribution of vitamins in the mammalian brain, we have developed a highly specific antiserum directed against retinoic acid with good affinity (10(-8) M), as evaluated by ELISA tests. In the rat brain, no immunoreactive fibers containing retinoic acid were detected. Cell bodies containing retinoic acid were only found in the hypothalamus. This work reports the first visualization and the morphological characteristics of cell bodies containing retinoic acid in the mammalian paraventricular hypothalamic nucleus and in the dorsal perifornical region, using an indirect immunoperoxidase technique. The restricted distribution of retinoic acid in the rat brain suggests that this vitamin could be involved in very specific physiological mechanisms.
为了增加我们对哺乳动物大脑中维生素分布的了解，我们开发了一种针对视黄酸的高特异性抗血清，其亲和力很好（10(-8) M），如 ELISA 测试所示。在大鼠脑中，没有检测到含有视黄酸的免疫反应纤维。含有视黄酸的细胞体仅在下丘脑发现。这项工作首次使用间接免疫过氧化物酶技术报告了含有视黄酸的细胞体在哺乳动物室旁下丘脑核和背侧peri 穹窿区域中的可视化和形态特征。视黄酸在大鼠脑中的有限分布表明，这种维生素可能参与非常特定的生理机制。
