口腔上皮内肿瘤组织嗜酸性粒细胞增多可能提示浸润。

Tissue eosinophilia in oral intraepithelial neoplasia as a probable indicator of invasion.

机构信息

Oral Medicine and Oral and Maxillofacial Pathology Unit, Laboratory of Oral & Maxillofacial Pathology, Division of Oral Maxillofacial Surgery, Department of Surgery, University Hospitals of Geneva, University of Geneva, Geneva, Switzerland.

Department of Pathology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.

出版信息

Oral Dis. 2018 Mar;24(1-2):103-108. doi: 10.1111/odi.12789.

DOI:10.1111/odi.12789

PMID:29480618

Abstract

OBJECTIVE

To investigate the presence of eosinophils in oral intraepithelial neoplasia (OIN) and oral squamous cell carcinoma (OSCC) lesions and its relation to invasion.

SUBJECTS AND METHODS

Ninety-nine oral biopsies were selected and subdivided into the following: OIN-1(16 cases), OIN-2 (18 cases), OIN-3 (17 cases), microinvasive OSCC (10 cases), non-metastatic OSCC (22 cases) and metastatic OSCC (16 cases). The tissue eosinophilia was evaluated histologically in slices stained with haematoxylin and eosin.

RESULTS

Eosinophil distribution was associated with diagnosis severity (p < .01). A significant difference was found between OIN-3 or microinvasive OSCC and non-metastatic or metastatic OSCC. Stromal invasion threshold was 7 eos/10 high power field (hpf) (96.1% specificity and 62.5% sensitivity). Eosinophils were absent in OIN-1; in OIN-2, two cases were positive. In OIN-3, five cases showed tissue eosinophilia, four of which had ≥3 eos/hpf or ≥7 eos/10 hpf. Three cases were suspected of invasion; two had a previous history of OSCC with elevated eosinophil infiltrate. In microinvasive OSCC, the four positive cases presented ≥3 eos/hpf and ≥7 eos/10 hpf. Although not significantly different, non-metastatic invasive OSCC had a higher number of cases (68.2%) with ≥22 eos/10 hpf contrasting with 50% in metastatic OSCC.

CONCLUSION

Our results suggest that eosinophils can be considered an indicator of invasion in OIN, helping in cases of difficult diagnosis.

摘要

目的

研究嗜酸性粒细胞在口腔上皮内瘤变（OIN）和口腔鳞状细胞癌（OSCC）病变中的存在及其与侵袭的关系。

方法

选择了 99 例口腔活检组织，分为以下几类：OIN-1（16 例）、OIN-2（18 例）、OIN-3（17 例）、微侵袭性 OSCC（10 例）、非转移性 OSCC（22 例）和转移性 OSCC（16 例）。用苏木精和伊红染色的切片进行组织嗜酸性粒细胞评估。

结果

嗜酸性粒细胞的分布与诊断严重程度有关（p<0.01）。在 OIN-3 或微侵袭性 OSCC 与非转移性或转移性 OSCC 之间发现了显著差异。间质侵袭阈值为 7 个嗜酸性粒细胞/10 高倍视野（96.1%的特异性和 62.5%的敏感性）。OIN-1 中没有嗜酸性粒细胞；OIN-2 中有两例阳性。在 OIN-3 中，有五例组织嗜酸性粒细胞增多，其中四例≥3 个嗜酸性粒细胞/ hpf 或≥7 个嗜酸性粒细胞/10 hpf。有三个病例疑似侵袭；其中两个有先前的 OSCC 病史，嗜酸性粒细胞浸润增加。在微侵袭性 OSCC 中，四个阳性病例表现出≥3 个嗜酸性粒细胞/ hpf 和≥7 个嗜酸性粒细胞/10 hpf。虽然没有显著差异，但非转移性侵袭性 OSCC 中≥22 个嗜酸性粒细胞/10 hpf 的病例数（68.2%）高于转移性 OSCC 的 50%。