真菌在水中的保存（卡斯泰拉尼法）：20年。
Preservation of fungi in water (Castellani): 20 years.
作者信息
Hartung de Capriles C, Mata S, Middelveen M
机构信息
Sección de Micología, Instituto de Medicina Tropical, Universidad Central de Venezuela, Caracas.
出版信息
Mycopathologia. 1989 May;106(2):73-9. doi: 10.1007/BF00437084.
Five-hundred ninety-four strains of fungi were studied. They were found being preserved with Castellani's method with distilled water during 1 to 20 years. 62% of the strains (n=368) did grow when subcultured and maintained their main morphological features. 90% of the 20 years old strains of different species were viable. It is argued that the technique of introduction of the strains into the water and their optimal condition will determine survival. The Castellani's method is recommended as easy, cheap and satisfactory for preservation of most species of fungi.
对594株真菌进行了研究。发现它们采用 Castellani 方法用蒸馏水保存了1至20年。62% 的菌株（n = 368）在传代培养时能够生长并保持其主要形态特征。不同物种的20年陈旧菌株中有90% 仍具有活力。有人认为，将菌株引入水中的技术及其最佳条件将决定其存活情况。推荐 Castellani 方法，因为它简便、廉价且对于大多数真菌物种的保存效果令人满意。
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