丝状真菌、酵母和一些需氧放线菌的菌种保藏于无菌蒸馏水中。
Storage of stock cultures of filamentous fungi, yeasts, and some aerobic actinomycetes in sterile distilled water.
作者信息
McGinnis M R, Padhye A A, Ajello L
出版信息
Appl Microbiol. 1974 Aug;28(2):218-22. doi: 10.1128/am.28.2.218-222.1974.
Abstract
Castellani's procedure for maintaining cultures of filamentous fungi and yeasts in sterile distilled water was evaluated. Four hundred and seventeen isolates of 147 species belonging to 66 genera of filamentous fungi, yeasts, and aerobic actinomycetes were maintained in sterile distilled water at room temperature over periods ranging from 12 to 60 months in four independent experiments. Of the 417 cultures, 389 (93%) survived storage in sterile distilled water. The selection of good sporulating cultures and sufficient inoculum consisting of spores and hyphae suspended in sterile distilled water were the most important factors influencing survival in water over a longer period of time. The technique was found to be simple, inexpensive, and reliable.
摘要
对卡斯特拉尼氏在无菌蒸馏水中保存丝状真菌和酵母培养物的方法进行了评估。在四项独立实验中，将属于丝状真菌、酵母和好氧放线菌66个属的147个物种的417个分离株在室温下保存在无菌蒸馏水中12至60个月。在这417个培养物中，389个（93%）在无菌蒸馏水中保存后存活下来。选择产孢良好的培养物以及由悬浮在无菌蒸馏水中的孢子和菌丝组成的足够接种物是影响在水中较长时间存活的最重要因素。该技术简单、廉价且可靠。
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