Shapiro S, Sartorius C, Sanders S, Clark S
Department of Neurosurgery, Indiana University School of Medicine, Indianapolis.
Neurosurgery. 1989 Oct;25(4):584-8; discussion 588-9. doi: 10.1097/00006123-198910000-00012.
End-to-side, laser-assisted vascular anastomosis (LAVA) using a Nd: YAG laser was successfully performed on rat carotid arteries. A midline neck incision allowed isolation and approximation of both carotid arteries in an end-to-side fashion using four 10-0 nylon stay sutures. The laser parameters used for vessel fusion were 0.3-second 5-W pulses at a spot size of 600 microns. Anastomoses were analyzed at various time intervals from 1 day to 6 months by angiogram and histological examination. The anastomotic patency was 86%. Aneurysm formation occurred in 23%. Histological examination revealed an acute/subacute transmural injury both at the anastomotic site and several hundred microns away, with delayed re-endothelialization and some attempt at muscular and elastic regeneration. Histological assessment of the aneurysms demonstrated a total loss of the internal elastic lamina and muscularis. A brief discussion comparing Nd: YAG LAVA to other LAVA techniques follows.
使用钕钇铝石榴石激光进行的端侧激光辅助血管吻合术（LAVA）在大鼠颈动脉上成功实施。通过颈部正中切口，使用四根10-0尼龙定位缝线以端侧方式分离并靠近双侧颈动脉。用于血管融合的激光参数为光斑尺寸600微米、0.3秒5瓦脉冲。在术后1天至6个月的不同时间间隔，通过血管造影和组织学检查对吻合口进行分析。吻合口通畅率为86%。动脉瘤形成发生率为23%。组织学检查显示，吻合部位及数百微米外均出现急性/亚急性透壁损伤，内皮化延迟，肌肉和弹性组织有一定再生尝试。动脉瘤的组织学评估显示内弹性膜和肌层完全缺失。接下来是对钕钇铝石榴石激光辅助血管吻合术与其他激光辅助血管吻合术技术的简要比较讨论。
