Christesen Danielle, Yang Ying Ting, Somers Jason, Robin Charles, Sztal Tamar, Batterham Philip, Perry Trent
School of Biosciences, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Victoria 3010, Australia.
School of Biosciences, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Victoria 3010, Australia
G3 (Bethesda). 2017 Feb 9;7(2):467-479. doi: 10.1534/g3.116.037333.
In larvae, the ring gland (RG) is a control center that orchestrates major developmental transitions. It is a composite organ, consisting of the prothoracic gland, the corpus allatum, and the corpora cardiaca, each of which synthesizes and secretes a different hormone. Until now, the RG's broader developmental roles beyond endocrine secretion have not been explored. RNA sequencing and analysis of a new transcriptome resource from wandering third instar larval RGs has provided a fascinating insight into the diversity of developmental signaling in this organ. We have found strong enrichment of expression of two gene pathways not previously associated with the RG: immune response and fatty acid metabolism. We have also uncovered strong expression for many uncharacterized genes. Additionally, RNA interference against RG-enriched cytochrome p450s and produced a lethal ecdysone deficiency and a juvenile hormone deficiency, respectively, flagging a critical role for these genes in hormone synthesis. This transcriptome provides a valuable new resource for investigation of roles played by the RG in governing insect development.
在幼虫中，环腺（RG）是协调主要发育转变的控制中心。它是一个复合器官，由前胸腺、咽侧体和心侧体组成，每个部分都合成并分泌一种不同的激素。到目前为止，尚未探索环腺在内分泌分泌之外更广泛的发育作用。对来自三龄漫游幼虫环腺的新转录组资源进行RNA测序和分析，为了解该器官发育信号的多样性提供了有趣的见解。我们发现了两条以前与环腺无关的基因途径的强烈表达富集：免疫反应和脂肪酸代谢。我们还发现许多未表征基因有强烈表达。此外，针对环腺富集的细胞色素p450进行RNA干扰，分别导致致命的蜕皮激素缺乏和保幼激素缺乏，这表明这些基因在激素合成中起关键作用。这个转录组为研究环腺在控制昆虫发育中所起的作用提供了宝贵的新资源。
