Srinivasan Sathiyaraj, Lim Sangyong, Lim Jae-Hyun, Jung Hee-Young, Kim Myung Kyum
Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 01797, Korea.
Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea.
J Microbiol Biotechnol. 2017 Mar 28;27(3):535-541. doi: 10.4014/jmb.1609.09002.
Two Gram-staining-negative, red-pinkish, coccus-shaped, non-motile, and aerobic bacterial strains, designated Ant21 and Ant22, were isolated from the Antarctic coastal sea water. Strains Ant21 and Ant22 showed UVC and gamma radiation resistance. Phylogenetic analyses based on 16 rRNA gene sequences determined that these strains belong to the genus . Through the analyses of the 16 rRNA gene sequences, strains Ant21 and Ant22 were found to have 97.7% and 97.8% similarity to DSM 12784 and 97.0% and 97.2% similarity to AA-1444, respectively. The sequence similarity with the type strains of other species was less than 96.9% for both strains. Strains Ant21 and Ant22 shared relatively high 16 rRNA gene sequence similarity (99.3%) and had a closely related DNA reassociation value of 84 ± 0.5%. Meanwhile, they showed a low level of DNA-DNA hybridization (<30%) with other closely related species of the genus . The two strains also showed typical chemotaxonomic features for the genus , in terms of the major polar lipid (phosphoglycolipid) and the major fatty acids (C, C6/7, iso-C , and iso-C). They grew at temperatures between 4°C and 30°C and at pH values of 6.0-8.0. Based on the physiological characteristics, the 16S rRNA gene sequence analysis results, and the low DNA-DNA reassociation level with , strains Ant21 (= KEMB 9004-167 =JCM 31436) and Ant22 (KEMB 9004-168 =JCM 31437) represent novel species belonging to the genus , for which the name is proposed.
从南极沿海水域分离出两株革兰氏染色阴性、呈红粉色、球菌状、无运动性且需氧的细菌菌株，分别命名为Ant21和Ant22。Ant21和Ant22菌株表现出对紫外线C和γ辐射的抗性。基于16 rRNA基因序列的系统发育分析确定这些菌株属于 属。通过对16 rRNA基因序列的分析，发现Ant21和Ant22菌株与DSM 12784的相似性分别为97.7%和97.8%，与AA - 1444的相似性分别为97.0%和97.2%。这两个菌株与其他 属物种的模式菌株的序列相似性均低于96.9%。Ant21和Ant22菌株具有相对较高的16 rRNA基因序列相似性（99.3%），并且具有紧密相关的DNA重结合值84 ± 0.5%。同时，它们与 属的其他密切相关物种的DNA - DNA杂交水平较低（<30%）。就主要极性脂质（磷酸糖脂）和主要脂肪酸（C、C6/7、异C 和异C）而言，这两个菌株还表现出该 属典型的化学分类特征。它们在4°C至30°C的温度和6.0 - 8.0的pH值下生长。基于生理特征、16S rRNA基因序列分析结果以及与 的低DNA - DNA重结合水平，Ant21（= KEMB 9004 - 167 = JCM 31436）和Ant22（KEMB 9004 - 168 = JCM 31437）菌株代表了 属的新物种，为此提出了名称 。
