从幼儿粪便中分离高产胞外多糖的魏斯氏菌 VP30 及其特性研究。
Isolation and characterization of high exopolysaccharide-producing Weissella confusa VP30 from young children's feces.
机构信息
Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, Seoul, 08826, South Korea.
Research Center, BIFIDO Co., Ltd., Hongcheon, 25117, South Korea.
出版信息
Microb Cell Fact. 2019 Jun 13;18(1):110. doi: 10.1186/s12934-019-1158-1.
BACKGROUND
Lactic acid bacteria (LAB) are known to have a significant ability to colonize the human intestinal tract and adhere to the surface of intestinal epithelial cells. Among the various lactic acid bacteria, exopolysaccharide (EPS) producing strains are known to provide a variety of health benefits for their hosts (e.g. anti-inflammatory, antioxidant, antitumor and stress tolerant effects). Recently, EPSs and EPS-producing lactic acid cultures have gained interest within the food industry and are playing important roles as biothickeners and texturizing agents due to their hydrocolloidal nature. In this study, 156 lactic acid bacterial strains isolated from fecal samples of healthy young children were screened and evaluated for active EPS-production capability.
RESULTS
Among the various human origin lactic acid flora isolated, Weissella confusa VP30 showed the highest EPS productivity and its EPS producing properties were characterized under various cultural conditions in this research. To document the safety of W. confusa VP30, antibiotic resistance, hemolytic, and ammonia production properties were evaluated in addition. No significant negative results were observed. The maximum EPS production by W. confusa VP30 was 59.99 ± 0.91 g/l after 48 h of cultivation in media containing 10% sucrose, far exceeding EPS production by other bacterial strains reported elsewhere. Based on gel permeation chromatography (GPC), the molecular weight of EPS produced by W. confusa VP30 was 3.8 × 10 Da. Structural analysis of the released EPS fraction by C and H nuclear magnetic resonance (NMR) spectroscopy revealed that W. confusa VP30 can produce dextran with glucose units linked with 96.5% α (1 → 6) glycosidic bonds and 3.5% α (1 → 3) branches.
CONCLUSION
The high EPS production capability and safety of W. confusa VP30 justify food industry consideration of this cell strain for further evaluation and potential industrial use.
背景
众所周知，乳酸菌（LAB）具有在人体肠道中定植和黏附在肠上皮细胞表面的显著能力。在各种乳酸菌中，已发现具有多种健康益处的胞外多糖（EPS）产生菌株，例如抗炎、抗氧化、抗肿瘤和抗应激作用。最近，EPS 和 EPS 产生的乳酸菌培养物在食品工业中引起了关注，由于其亲水性，它们作为生物增稠剂和质构化剂发挥着重要作用。在这项研究中，从健康幼儿粪便样本中分离出 156 株乳酸菌，对其活性 EPS 产生能力进行了筛选和评估。
结果
在所分离的各种人类来源的乳酸菌中，魏斯氏菌属（Weissella）的 VP30 表现出最高的 EPS 生产力，并且在本研究中在各种培养条件下对其 EPS 产生特性进行了表征。为了记录魏斯氏菌属 VP30 的安全性，除了评估其抗生素耐药性、溶血和氨产生特性外，还进行了评估。没有观察到明显的负面结果。魏斯氏菌属 VP30 在含有 10%蔗糖的培养基中培养 48 小时后，最大 EPS 产量为 59.99±0.91g/L，远远超过了其他细菌菌株在其他地方报道的 EPS 产量。基于凝胶渗透色谱（GPC），魏斯氏菌属 VP30 产生的 EPS 的分子量为 3.8×10 Da。通过 C 和 H 核磁共振（NMR）光谱对释放的 EPS 级分进行结构分析表明，魏斯氏菌属 VP30 可以产生葡萄糖单位以 96.5%α（1→6）糖苷键和 3.5%α（1→3）支链连接的右旋糖酐。
结论
魏斯氏菌属 VP30 的高 EPS 生产能力和安全性证明了该细胞株值得食品行业进一步评估和潜在的工业用途。
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