Gnanasekaran Gopalsamy, Na Eun Jung, Chung Han Young, Kim Suyeon, Kim You-Tae, Kwak Woori, Kim Heebal, Ryu Sangryeol, Choi Sang Ho, Lee Ju-Hoon
Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.
Food-borne Pathogen Omics Research Center (FORC), Seoul National University, Seoul 08826, Republic of Korea.
J Microbiol Biotechnol. 2017 Feb 28;27(2):262-270. doi: 10.4014/jmb.1611.11048.
is a well-known foodborne pathogen causing gastrointestinal infections worldwide. The strain FORC_002 was isolated from the gill of flatfish (plaice) and its genome was sequenced. The genomic DNA consists of 4,837,317 bp with a GC content of 47.1%, and is predicted to contain 4,221 open reading frames, 81 tRNA genes, and 26 rRNA genes. Interestingly, genomic analysis revealed pathogenesis and host immune evasion-associated genes encoding guanylate cyclase (Yst), invasin (Ail and Inv), outer membrane protein (Yops), autotransporter adhesin A (YadA), RTX-like toxins, and a type III secretion system. In particular, guanylate cyclase is a heat-stable enterotoxin causing -associated diarrhea, and RTX-like toxins are responsible for attachment to integrin on the target cell for cytotoxic action. This genome can be used to identify virulence factors that can be applied for the development of novel biomarkers for the rapid detection of this pathogen in foods.
是一种在全球范围内引起胃肠道感染的著名食源性病原体。FORC_002菌株是从比目鱼（鲽鱼）的鳃中分离出来的，并对其基因组进行了测序。基因组DNA由4,837,317个碱基对组成，GC含量为47.1%，预计包含4,221个开放阅读框、81个tRNA基因和26个rRNA基因。有趣的是，基因组分析揭示了与发病机制和宿主免疫逃避相关的基因，这些基因编码鸟苷酸环化酶（Yst）、侵袭素（Ail和Inv）、外膜蛋白（Yops）、自转运黏附素A（YadA）、RTX样毒素和III型分泌系统。特别是，鸟苷酸环化酶是一种引起相关腹泻的热稳定肠毒素，RTX样毒素负责与靶细胞上的整合素结合以发挥细胞毒性作用。该基因组可用于鉴定毒力因子，这些毒力因子可用于开发新型生物标志物，以快速检测食品中的这种病原体。
